LOCUS       sequence-277666-        2597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1582
FEATURES             Location/Qualifiers
     source          1..2597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagaacca acgctcatca cctctgtcgt tccccagtgt tccacaagaa
       61 gaaaccttac gtcaggcccc tgctggactc ccccgagaaa ctctgttcca atcccgcgtt
      121 cttcctccca aagaaattcc ttctttgtct cccaccattc cccgtcaagg ctccctgccc
      181 caaacttcca gtgctcccaa gcaagagact tctggccgga tgccacatgt gctccagaag
      241 ggaccctcac tcctgtgttc tgccgcttct gagcaagaga cttctctcca gggccccctg
      301 gcttcccagg aagggaccca gtatccaccc ccagctgctg ctgaacaaga agtctccctt
      361 ctctcccact ccccccacca ccaggaagcc cccgttcact cccctgaagc tcctgagaaa
      421 gaccccctga ccctttcccc aacagttccc gagactgaca tggacccgct gctccagagc
      481 ccggtttccc aaaaggacac ccctttccag atctcttctg cagtccagaa ggaacagccg
      541 ctccccacgg cagagatcac ccgcttggct gtgtgggctg ccgtccaagc agtggagagg
      601 aagctggagg cccaggccat gaggctactg accctggaag gcaggacggg gacaaatgaa
      661 aagaagatag ccgactgcga gaagacagcc gtggagttcg cgaaccatct ggagagcaag
      721 tgggtcgtgt tggggaccct gctgcaggag tatgggctgc tgcagaggcg gctggagaac
      781 atggagaacc tgctgaaaaa cagaaatttc tggatcctgc ggctgccccc cggcagcaat
      841 ggagaagttc ccaaggtccc tgtcacattt gatgatgttg ctgtgcactt ctcggagcag
      901 gagtggggaa acctgtctga gtggcagaag gagctctaca agaacgtgat gaggggcaac
      961 tacgagtccc tggtttccat ggactatgca atttccaaac cagacctcat gtcacagatg
     1021 gagcgcgggg agcggcccac catgcaggag caggaagact ctgaggaggg cgaaacgccg
     1081 acagatccca gtgctgcgca cgatgggatc gtgattaaga tcgaggtaca gaccaacgac
     1141 gagggctcag aaagtttgga gacacctgag cccctgatgg gacaggtgga agagcacggc
     1201 ttccaggact cagagctggg tgacccctgt ggggaacagc cagacctgga catgcaggag
     1261 ccagagaaca cgctggagga gtccacggaa ggctccagcg agttcagcga actgaagcag
     1321 atgctggtgc agcagaggaa ctgcacggag gggatcgtga tcaagacaga ggaacaagac
     1381 gaggaggaag aagaggagga ggaggatgag ctgccgcagc acttgcaatc ccttgggcag
     1441 ctgtccggga gatatgaggc cagtatgtac cagaccccgc tgcccgggga gatgtccccc
     1501 gagggcgagg agagcccccc gcccctgcag ctaggaaacc ccgcagtgaa aaggctggcg
     1561 ccctccgtgc acggtgagcg gcacctgagc gagaaccgcg gggcctcgag ccagcagcag
     1621 cggaaccggc gcggcgagcg gcccttcaca tgcatggagt gcggcaagag cttccgcctg
     1681 aagatcaacc tcatcatcca ccagcgcaac cacatcaagg aggggcccta cgagtgcgcc
     1741 gaatgcgaga tcagcttccg gcacaagcaa cagctcacgc tgcaccagcg catccaccgc
     1801 gtgcgcggag gctgcgtctc acccgaacgc gggcccacgt tcaaccccaa gcacgcgctc
     1861 aagccgcgtc ccaagtcacc cagctctggt agcggcggcg gtggccctaa gccctacaag
     1921 tgccccgagt gcgacagcag cttcagccac aagtccagcc tgaccaaaca ccagatcacg
     1981 cacacgggtg agcggcccta cacgtgcccc gagtgcaaga agagcttccg cctgcacatc
     2041 agcttggtga tccatcagcg cgtgcacgcg ggcaagcatg aggtctcctt catctgcagc
     2101 ctgtgcggca agagcttcag ccgcccctcg cacctgctgc gccaccagcg gactcacaca
     2161 ggcgagcggc ccttcaagtg ccccgagtgc gagaagagct tcagcgagaa gtccaagctc
     2221 accaaccact gccgcgtgca ctcgcgcgag cggccgcacg cctgccccga gtgcggcaag
     2281 agcttcatcc gcaagcacca cctcctggaa caccggcgca tccacacagg cgagcggccc
     2341 taccactgcg ccgagtgcgg caagcgcttc acgcagaagc atcacctgct ggagcaccag
     2401 cgcgcgcaca cgggcgagcg gccctacccc tgcacgcact gcgccaagtg cttccgctac
     2461 aagcagtcgc tcaagtacca cctgcggacc cacacgggcg agtagtaatg aactagtccg
     2521 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgattcctaa
     2581 gccggtcata tcatgac
//
