LOCUS       sequence-27771-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Kota J, Chivukula RR, O'Donnell KA, Wentzel EA, Montgomery CL, Hwang
            HW, Chang TC, Vivekanandan P, Torbenson M, Clark KR, Mendell JR, 
            Mendell JT
  TITLE     Therapeutic microRNA delivery suppresses tumorigenesis in a murine 
            liver cancer model.
  JOURNAL   Cell. 2009 Jun 12. 137(6):1005-17.
  PUBMED    19524505
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 12. 137(6):1005-17."
COMMENT     Sequence Label: scAAV GFP
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    21..101
                     /label=MCS
                     /note="multiple cloning site"
     intron          114..317
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(137..157)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ccttgctcac catggtggcg accggtggat cccgggcccg cggtaccgtc gactgcagaa
       61 ttcgaagctt gagctcgaga tctgagtccg gtagcgctag cttttcacga cacctgaaat
      121 ggaagaaaaa aactttgaac cactgtctga ggcttgagaa tgaaccaaga tccaaactca
      181 aaaagggcaa attccaagga gaattacatc aagtgccaag ctggcctaac ttcagtctcc
      241 acccactcag tgtggggaaa ctccatcgca taaaacccct ccccccaacc taaagacgac
      301 gtactccaaa agctcgagaa ctaatcgagg tgcctggacg gcgcccggta ctccgtggag
      361 tcacatgaag cgacggctga ggacggaaag gcccttttcc tttgtgtggg tgactcaccc
      421 gcccgctctc ccgagcgccg cgtcctccat tttgagctcc ctgcagcagg gccgggaagc
      481 ggccatcttt ccgctcacgc aactggtgcc gaccgggcca gccttgccgc ccagggcggg
      541 gcgatacacg gcggcgcgag gccaggcacc agagcaggcc ggccagcttg agactacccc
      601 cgtccgattc tcggtggccg cgctcgcagg ccccgcctcg ccgaacatgt gcgctgggac
      661 gcacgggccc cgtcgccgcc cgcgggaacc acacacggca cttacctgtg ttctggcggc
      721 aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc
      781 accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca
      841 ccttctctag gcaccggttc aattgccg
//
