LOCUS       sequence-277813-        1274 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1938
FEATURES             Location/Qualifiers
     source          1..1274
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgcaaag gtacgacgat ctaccccatt acgggggcat ggatggagta
       61 ggcatcccct ccacgatgta tggggacccg catgcagcca ggtccatgca gccggtccac
      121 cacctgaacc acgggcctcc tctgcactcg catcagtacc cgcacacagc tcataccaac
      181 gccatggccc ccagcatggg ctcctctgtc aatgacgctt taaagagaga taaagatgcc
      241 atttatggac accccctctt ccctctctta gcactgattt ttgagaaatg tgaattagct
      301 acttgtaccc cccgcgagcc gggggtggcg ggcggggacg tctgctcgtc agagtcattc
      361 aatgaagata tagccgtgtt cgccaaacag attcgcgcag aaaaacctct attttcttct
      421 aatccagaac tggataactt gatgattcaa gccatacaag tattaaggtt tcatctattg
      481 gaattagaga aggtacacga attatgtgac aatttctgcc accggtatat tagctgtttg
      541 aaagggaaaa tgcctatcga tttggtgata gacgatagag aaggaggatc aaaatcagac
      601 agtgaagata taacaagatc agcaaatcta actgaccagc cctcttggaa cagagatcat
      661 gatgacacgg catctactcg ttcaggagga accccaggcc cttccagcgg tggccacacg
      721 tcacacagtg gggacaacag cagtgagcaa ggtgatggct tggacaacag tgtagcttcc
      781 cccagcacag gtgacgatga tgaccctgat aaggacaaaa agcgtcacaa aaagcgtggc
      841 atctttccca aagtagccac aaatatcatg agggcgtggc tgttccagca tctaacacac
      901 ccttaccctt ctgaagaaca gaaaaagcag ttggcacaag acacgggact caccatcctt
      961 caagtgaaca attggtttat taatgcccgg agaagaatag tgcagcccat gatagaccag
     1021 tccaaccgag cagtaagtca aggaacacct tataatcctg atggacagcc catgggaggt
     1081 ttcgtaatgg acggtcagca acatatggga attagagcac caggacctat gagtggaatg
     1141 ggcatgaata tgggcatgga ggggcagtgg cactacatgt agtaatgaac tagtccgtaa
     1201 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga gtacattcgg
     1261 cctgtgggct catt
//
