LOCUS       sequence-27784-e         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-mVenus-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     201..222
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(271..288)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(309..418)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    434..458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(458..477)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(459..477)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(519..538)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg cgaagcactg
       61 caggccgtag ccgagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
      121 gctgatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
      181 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
      241 gaacagctcc tcgcccttgc tcacagaacc gtgatggtgg tgatggtgtg ccatggtata
      301 tctcctttga ttgtaaataa aatgtaattt acagtatagt attttaatta atatacaaat
      361 gatttgataa taattcttat ttaactataa tatattgtgt tgggttgaat taaaggtccc
      421 ggattaactc cggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt
      481 tcggtccgat ccaattcttt gccaaaatga tgagacagca caataaccag cacgttgccc
      541 aggagctgta ggaaaaagaa gaaggcatga acatggttag cagaggctct agagccgccg
      601 gtcacacgcc agaaagccga accccgccct gccccgt
//
