LOCUS       sequence-278044-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2208
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggtgct ggagagcggg gagcagggcg tgctgcagtg ggaccgcaag
       61 ctgagcgagc tgtcagagcc cggggacggc gaggccctca tgtaccacac gcacttctca
      121 gaacttctgg atgagttttc ccagaacgtc ttgggtcagc tcctgaatga tcctttcctc
      181 tcagagaaga gtgtgtcaat ggaggtggaa ccttccccga cgtccccggc gcctctcatc
      241 caggctgagc acagctactc cctgtgcgag gagcctcggg cccagtcgcc cttcacccac
      301 attaccacca gtgacagctt caatgacgat gaggtggaaa gtgagaaatg gtacctgtct
      361 acagacttcc cttcaacatc catcaagaca gagccagtta cagacgaacc acccccagga
      421 ctcgttccgt ctgtcactct gaccatcaca gccatctcca ccccgttgga aaaggaggaa
      481 cctcctctgg aaatgaacac tggggttgat tcctcgtgcc agaccattat tcctaaaatt
      541 aagctggagc ctcatgaagt ggatcagttt ctaaacttct ctcctaaaga aggtctgtct
      601 gccctccctg tgtccctttg ggttatggat atggtctctg ggtctacaga gagggaatat
      661 ggcgagagag ctgggatgag tttgtaccac agatgttgta gctggcttta tgaaatagct
      721 ctgttcttaa aaaataaaaa ttttgcttcc aaatagtaat gaactagtcc gtaacttgaa
      781 agtatttcga tttcttggct ttatatatct tgtggaaagg acgacggagt gccaatcggg
      841 ttagagtt
//
