LOCUS       sequence-278095-        1343 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2242
FEATURES             Location/Qualifiers
     source          1..1343
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagttccc tggcctgggg tccctgggga cctcagagcc cctcccccag
       61 tttgtggatc ctgctctggt gtcctccaca ccagaatcag gggttttctt cccctctggg
      121 cctgagggct tggatgcagc agcttcctcc actgccccga gcacagccac cgctgcagct
      181 gcggcactgg cctactacag ggacgctgag gcctacagac actccccagt ctttcaggtg
      241 tacccattgc tcaactgtat ggaggggatc ccagggggct caccatatgc cggctgggcc
      301 tacggcaaga cggggctcta ccctgcctca actgtgtgtc ccacccgcga ggactctcct
      361 ccccaggccg tggaagatct ggatggaaaa ggcagcacca gcttcctgga gactttgaag
      421 acagagcggc tgagcccaga cctcctgacc ctgggacctg cactgccttc atcactccct
      481 gtccccaata gtgcttatgg gggccctgac ttttccagta ccttcttttc tcccaccggg
      541 agccccctca attcagcagc ctattcctct cccaagcttc gtggaactct ccccctgcct
      601 ccctgtgagg ccagggagtg tgtgaactgc ggagcaacag ccactccact gtggcggagg
      661 gacaggacag gccactacct atgcaacgcc tgcggcctct atcacaagat gaatgggcag
      721 aacaggcccc tcatccggcc caagaagcgc ctgattgtca gtaaacgggc aggtactcag
      781 tgcaccaact gccagacgac caccacgaca ctgtggcgga gaaatgccag tggggatccc
      841 gtgtgcaatg cctgcggcct ctactacaag ctacaccagg tgaaccggcc actgaccatg
      901 cggaaggatg gtattcagac tcgaaaccgc aaggcatctg gaaaagggaa aaagaaacgg
      961 ggctccagtc tgggaggcac aggagcagcc gaaggaccag ctggtggctt tatggtggtg
     1021 gctgggggca gcggtagcgg gaattgtggg gaggtggctt caggcctgac actgggcccc
     1081 ccaggtactg cccatctcta ccaaggcctg ggccctgtgg tgctgtcagg gcctgttagc
     1141 cacctcatgc ctttccctgg acccctactg ggctcaccca cgggctcctt ccccacaggc
     1201 cccatgcccc ccaccaccag cactactgtg gtggctccgc tcagctcata gtaatgaact
     1261 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
     1321 tatcagaact tgccgctctc gtg
//
