LOCUS       sequence-278119-        1982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2255
FEATURES             Location/Qualifiers
     source          1..1982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagacca gagaatggac atttcttcaa ccatcagtga tttcatgtcc
       61 ccgggcccca ccgacctgct ttccagctct cttggtacca gtggtgtgga ttgcaaccgc
      121 aaacggaaag gcagctccac tgactaccaa gaaagcatgg acacagacaa agatgaccct
      181 catggaaggt tagaatatac agaacaccaa ggaaggataa aaaatgcaag ggaagctcac
      241 agtcagattg aaaagcggcg tcgggataaa atgaacagtt ttatagatga attggcttct
      301 ttggtaccaa catgcaacgc aatgtccagg aaattagata aacttactgt gctaaggatg
      361 gctgttcagc acatgaaaac attaagaggt gccaccaatc catacacaga agcaaactac
      421 aaaccaactt ttctatcaga cgatgaattg aaacacctca ttctcagggc agcagatgga
      481 tttttgtttg tcgtaggatg tgaccgaggg aagatactct ttgtctcaga gtctgtcttc
      541 aagatcctca actacagcca gaatgatctg attggtcaga gtttgtttga ctacctgcat
      601 cctaaagata ttgccaaagt caaggagcag ctctcctcct ctgacaccgc accccgggag
      661 cggctcatag atgcaaaaac tggacttcca gttaaaacag atataacccc tgggccatct
      721 cgattatgtt ctggagcacg acgttctttc ttctgtagga tgaagtgtaa caggccttca
      781 gtaaaggttg aagacaagga cttcccctct acctgctcaa agaaaaaagc agatcgaaaa
      841 agcttctgca caatccacag cacaggctat ttgaaaagct ggccacccac aaagatgggg
      901 ctggatgaag acaacgaacc agacaatgag gggtgtaacc tcagctgcct cgtcgcaatt
      961 ggacgactgc attctcatgt agttccacaa ccagtgaacg gggaaatcag ggtgaaatct
     1021 atggaatatg tttctcggca cgcgatagat ggaaagtttg tttttgtaga ccagagggca
     1081 acagctattt tggcatattt accacaagaa cttctaggca catcgtgtta tgaatatttt
     1141 caccaagatg acataggaca tcttgcagaa tgtcataggc aagttttaca gacgagagaa
     1201 aaaattacaa ctaattgcta taaatttaaa atcaaagatg gttcttttat cacactacgg
     1261 agtcgatggt tcagtttcat gaacccttgg accaaggaag tagaatatat tgtctcaact
     1321 aacactgttg ttttagccaa cgtcctggaa ggcggggacc caaccttccc acagctcaca
     1381 gcatcccccc acagcatgga cagcatgctg ccctctggag aaggtggccc aaagaggacc
     1441 caccccactg ttccagggat tccaggggga acccgggctg gggcaggaaa aataggccga
     1501 atgattgctg aggaaatcat ggaaatccac aggataagag ggtcatcgcc ttctagctgt
     1561 ggctccagcc cattgaacat cacgagtacg cctccccctg atgcctcttc tccaggaggc
     1621 aagaagattt taaatggagg gactccagac attccttcca gtggcctact atcaggccag
     1681 gctcaggaga acccaggtta tccatattct gatagttctt ctattcttgg tgagaacccc
     1741 cacataggta tagacatgat tgacaacgac caaggatcaa gtagtcccag taatgatgag
     1801 gcagcaatgg ctgtcatcat gagcctcttg gaagcagatg ctggactggg tggccctgtt
     1861 gactttagtg acttgccatg gccgctgtag taatgaacta gtccgtaact tgaaagtatt
     1921 tcgatttctt ggctttatat atcttgtgga aaggacgact gctaaagttg ggctcagcta
     1981 ga
//
