LOCUS       sequence-27822-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-mCerulean-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..449
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 agggttctgg aagtggatcc ttggctacta cacttgaacg tattgagaag aactttgtca
       61 ttactgaccc aagattgcca gataatccca ttatattcgc gtccgatagt ttcttgcagt
      121 tgacagaata tagccgtgaa gaaattttgg gaagaaactg caggtttcta caaggtcctg
      181 aaactgatcg cgcgacagtg agaaaaatta gagatgccat agataaccaa acagaggtca
      241 ctgttcagct gattaattat acaaagagtg gtaaaaagtt ctggaacctc tttcacttgc
      301 agcctatgcg agatcagaag ggagatgtcc agtactttat tggggttcag ttggatggaa
      361 ctgagcatgt ccgagatgct gccgagagag agggagtcat gctgattaag aaaactgcag
      421 aaaatattga tgaggcggca aaagaactta tcaagtgtgt ggtggtggga gacggagctg
      481 taggtaaaac ttgcctactg atcagttaca caaccaatgc atttcctgga gaatatatcc
      541 ctactgtctt tgacaattat tctgccaatg ttatggtaga tggaaaaccg gtgaatctgg
      601 gcttatggga tacagctgga ctagaagatt atgacagatt acgcccccta tcctatccgc
      661 aaacagatgt gttcttaatt tgcttttccc ttgtgagtcc tgcatcattc caccacgtcc
      721 gtgcaaagtg gtatcctgag gtgcggcacc actgtcccaa cactcccatc atcctagtgg
      781 gaactaaact tgatctta
//
