LOCUS       sequence-27826-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pBabe-TetCMV-puro-mCerulean-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             117..134
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(183..204)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(444..463)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtcnatcgcc tggagacgcc atccacgctg ttttgacctc cnnnnnnnac accgggaccg
       61 atccagcctc cgcggcccca gatccgtaaa gtgaattcgg agcatatacc atggcacacc
      121 atcaccacca tcacggttct gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      181 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      241 agggcgatgc cacctacggc aagctgaccc tgaagttcat cancaccacc ggcaagctgc
      301 ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct
      361 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      421 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      481 tcgagggcga caccctggtg aaccgcatcg agctgaangg catcgacttc aaggaggacg
      541 gcaacatcct ggggcacaag ctggagtaca acgccctcnn cgacaacgnn nnnntcaccg
      601 ccgacaagca naagaacggc atcaaggcca acttcaagat ccgcnnnnnn atcganggac
      661 ggnagcgtgc agc
//
