LOCUS       sequence-278461-        2513 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2490
FEATURES             Location/Qualifiers
     source          1..2513
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2057..2068
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tggactttcc ccagcacagc cagcatgtct tggaacagct gaaccagcag
       61 cggcagctgg ggcttctctg tgactgcacc tttgtggtgg acggtgttca ctttaaggct
      121 cataaagcag tgctggcggc ctgcagcgag tacttcaaga tgctcttcgt ggaccagaag
      181 gacgtggtgc acctggacat cagtaacgcg gcaggcctgg ggcaggtgct ggagtttatg
      241 tacacggcca agctgagcct gagccctgag aacgtggatg atgtgctggc cgtggccact
      301 ttcctccaaa tgcaggacat catcacggcc tgccatgccc tcaagtcact tgctgagccg
      361 gctaccagcc ctgggggaaa tgcggaggcc ttggccacag aaggagggga caagagagcc
      421 aaagaggaga aggtggccac cagcacgctg agcaggctgg agcaggcagg acgcagcaca
      481 cccataggcc ccagcaggga cctcaaggag gagcgcggcg gtcaggccca gagtgcggcc
      541 agcggtgcag agcagacaga gaaagccgat gcgccccggg agccgccgcc tgtggagctc
      601 aagccagacc ccacgagtgg catggctgct gcagaagctg aggccgcttt gtccgagagc
      661 tcggagcaag aaatggaggt ggagcccgcc cggaaagggg aagaggagca aaaggagcaa
      721 gaggagcaag aggaggaggg cgcagggcca gctgaggtca aggaggaggg ttcccagctg
      781 gagaacggag aggcccccga ggagaacgag aatgaggagt cagcgggcac agactcgggg
      841 caggagctcg gctccgaggc ccggggcctg cgctcaggca cctacggcga ccgcacggag
      901 tccaaggcct acggctccgt catccacaag tgcgaggact gtgggaagga gttcacgcac
      961 acggggaact tcaagcggca catccgcatc cacacggggg agaagccctt ctcgtgccgg
     1021 gagtgcagca aggccttttc cgacccggcc gcgtgcaagg cccatgagaa gacgcacagc
     1081 cctctgaagc cctacggctg cgaggagtgc gggaagagct accgcctcat cagcctgctg
     1141 aacctgcaca agaagcggca ctcgggcgag gcgcgctacc gctgcgagga ctgcggcaag
     1201 ctcttcacca cctcgggcaa cctcaagcgc caccagctgg tgcacagcgg cgagaagccc
     1261 taccagtgcg actactgcgg ccgctccttc tccgacccca cttccaagat gcgccacctg
     1321 gagacccacg acacggacaa ggagcacaag tgcccacact gcgacaagaa gttcaaccag
     1381 gtagggaacc tgaaggccca cctgaagatc cacatcgctg acgggcccct caagtgccga
     1441 gagtgtggga agcagttcac cacctcaggg aacctgaagc ggcaccttcg gatccacagc
     1501 ggggagaagc cctacgtgtg catccactgc cagcgacagt ttgcagaccc cggcgctctg
     1561 cagcggcacg tccgcattca cacaggtgag aagccatgcc agtgtgtgat gtgcggtaag
     1621 gccttcaccc aggccagctc cctcatcgcc cacgtgcgcc agcacaccgg ggagaagccc
     1681 tacgtctgcg agcgctgcgg caagagattc gtccagtcca gccagttggc caatcatatt
     1741 cgccaccacg acaacatccg cccacacaag tgcagcgtgt gcagcaaggc cttcgtgaac
     1801 gtgggggacc tgtccaagca catcatcatt cacactggag agaagcctta cctgtgtgat
     1861 aagtgtgggc gtggcttcaa ccgggtagac aacctgcgct cccacgtgaa gaccgtgcac
     1921 cagggcaagg caggcatcaa gatcctggag cccgaggagg gcagtgaggt cagcgtggtc
     1981 actgtggatg acatggtcac gctggctacc gaggcactgg cagcgacagc cgtcactcag
     2041 ctcacagtgg tgccggtggg agctgcagtg acagccgatg agacggaagt cctgaaggcc
     2101 gagatcagca aagctgtgaa gcaagtgcag gaagaagacc ccaacactca catcctctac
     2161 gcctgtgact cctgtgggga caagtttctg gatgccaaca gcctggctca gcatgtgcga
     2221 atccacacag cccaggcact ggtcatgttc cagacagacg cggacttcta tcagcagtat
     2281 gggccaggtg gcacgtggcc tgccgggcag gtgctgcagg ctggggagct ggtcttccgc
     2341 cctcgcgacg gggctgaggg ccagcccgca ctggcagaga cctcccctac agctcctgaa
     2401 tgtcccccgc ctgccgagta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2461 tggctttata tatcttgtgg aaaggacgat cctatctgat ccgaacgcac taa
//
