LOCUS       sequence-278579-        5846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0125
FEATURES             Location/Qualifiers
     source          1..5846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggacgg aaagggagac gccgccgctg tcgccggggc tggggctgag
       61 gctccggcgg tagcgggagc cggagatgga gtcgagactg agtccatggt tcggggtcat
      121 cgccccgtat ctccagcgcc gggagcctcg ggactgcggc cgtgtctgtg gcagctggag
      181 acagagctga gggagcaaga ggtgtcggag gtctcatctt tgaactactg ccggagcttc
      241 tgccagacct tattgcaata tgcaagcaac aagaatgcat cagaacatat tgtgtatctt
      301 ctggaggtat atcgacttgc catccaaagc tttgccagtg cacgtccata cttaactact
      361 gaatgtgaag atgtcctctt agtgcttggc agattagtac tgagttgttt cgaattactg
      421 ctttcagtgt ctgaaagtga actgccatgt gaagtctggc taccattcct tcagtctcta
      481 caggagtcac atgatgcatt attggaattt gggaataata acctacaaat attggttcat
      541 gttaccaagg aaggggtgtg gaaaaaccca gttcttctta aaattctgtc tcaacagcca
      601 gtagaaacgg aggaagtcaa taaattgatt gcacaagaag gaccttcctt tctgcaaatg
      661 cgaataaaac atttgttgaa atctaactgc atcccccagg ctactgcttt atcaaaacta
      721 tgtgcagaat ctaaagaaat ttcaaatgtg tcatcttttc agcaagccta tatcacatgt
      781 ttatgttcta tgctccctaa tgaagatgct attaaggaga ttgcaaaggt cgactgcaag
      841 gaagtactag acatcatttg taatctggaa tctgaggggc aggataacac agcatttgtt
      901 ctttgtacga cttaccttac ccagcagctc caaactgcaa gtgtatattg ttcttgggaa
      961 ctgactcttt tttggagtaa actgcaaaga agaattgacc cttctttaga tacttttttg
     1021 gagcgctgtc gtcagtttgg tgtcatagct aaaacgcagc agcatttatt ttgcctcatt
     1081 agagttatac aaactgaagc acaagatgct ggtcttgggg tgtcaatttt actgtgtgtc
     1141 agagctcttc aactcagatc aagtgaagat gaggaaatga aggcatcagt ttgtaaaaca
     1201 attgcctgtc ttttaccaga agatttagaa gttagacgag cctgtcagct tacagaattc
     1261 ttaattgaac ccagtttgga tggatttaat atgttagaag aactatattt gcaaccagat
     1321 caaaaatttg atgaagaaaa tgcaccggtt ccaaattctc ttcgatgtga gctcttacta
     1381 gctttaaaag cccactggcc ttttgatcct gagttttggg actggaaaac tttaaaacga
     1441 cactgccacc aacttttagg acaagaagcc tcagattctg atgatgattt aagtggctat
     1501 gaaatgtcca ttaatgacac agatgtttta gagtcatttc tcagtgacta tgatgagggt
     1561 aaagaagata aacaatatag aagaagagat ttgacagatc agcataagga gaaaagagac
     1621 aaaaaaccta ttggctcttc tgaaagatat cagaggtggc ttcagtacaa gtttttctgt
     1681 ttgttatgta agcgggaatg tatagaggct agaattcttc atcattctaa gatgcatatg
     1741 gaagatggaa tttacacctg tccagtttgt attaaaaaat ttaagagaaa agaaatgttt
     1801 gttcctcatg tgatggagca tgttaaaatg ccaccaagca gaagggaccg ctctaaaaag
     1861 aaattactgt taaaaggctc tcaaaagggt atttgtccta agagcccctc tgcaatccca
     1921 gagcaaaacc attcattgaa tgaccaagcc aaaggagagt ctcatgaata tgtcacattc
     1981 agcaaattag aagattgcca cctgcaagac agagatttgt atccatgtcc cggtacagac
     2041 tgttcccgtg tgtttaagca atttaaatac ttaagtgtgc atcttaaagc tgaacaccaa
     2101 aataatgatg aaaatgccaa gcactacttg gatatgaaaa atagaagaga gaagtgtact
     2161 tactgtcgac gacattttat gtctgctttt caccttcgag agcacgaaca agtgcattgt
     2221 gggcctcagc cttatatgtg tgtatctata gattgctatg ctaggtttgg atcagtaaat
     2281 gaactactta accataaaca aaagcatgac gatctgcgtt acaaatgtga attaaatggc
     2341 tgtaatattg ttttcagtga cttgggacag ctttaccacc atgaagcaca acactttagg
     2401 gatgcatctt acacatgcaa cttccttggc tgtaaaaagt tctattactc caaaattgaa
     2461 taccagaatc acctctcaat gcataatgtt gaaaattcaa atggagacat aaagaaatca
     2521 gtgaaacttg aggagtctgc aacaggtgaa aagcaagatt gtattaatca gccccatcta
     2581 cttaaccaaa ctgataaatc acatttacct gaagatcttt tctgtgcaga atcagctaat
     2641 tctcaaatag atacagaaac tgcagaaaac ctgaaagaaa acagtgacag taattctagt
     2701 gatcagttaa gtcatagctc ttcagcttca atgaatgaag agctaattga cacactagat
     2761 cactctgaaa ctatgcagga tgtattgtta tctaatgaga aagtctttgg gccctccagt
     2821 ttaaaagaaa aatgttccag tatggcagtt tgttttgacg ggactaagtt tacctgtggt
     2881 tttgatggct gtggttccac atacaaaaat gcaagaggaa tgcagaaaca tttacggaag
     2941 gttcatccat accatttcaa gcccaaaaag ataaagacga aagatctgtt tccctctttg
     3001 ggtaatgaac ataatcagac aactgaaaag ttggatgcag aacctaaacc ctgctcagat
     3061 acaaacagtg actccccaga tgaaggtcta gatcacaata ttcacattaa atgtaaacga
     3121 gaacatcaag gttattcctc agaatcctcc atttgtgctt ctaaaaggcc ctgtacagag
     3181 gataccatgt tggaacttct gttacgcttg aaacatttaa gcttgaaaaa ctcaataaca
     3241 catggatctt tctcagggtc attgcagggg tacccatcca gtggtgctaa gtctcttcag
     3301 tcagtttcat ctatctcaga ccttaatttt cagaatcaag atgaaaacat gccaagtcag
     3361 taccttgcac agttggcggc taagccgttt ttctgtgagc ttcaaggatg caaatatgaa
     3421 tttgtgacca gagaggctct gttaatgcat tatcttaaaa agcataatta ttcaaaagaa
     3481 aaagtccttc agttaaccat gttccaacat cggtattccc catttcagtg tcatatttgc
     3541 caaaggtcat ttacaagaaa aacacacctt aggattcatt ataaaaataa acatcaaatt
     3601 ggcagtgaca gagcaactca caaactatta gataatgaaa agtgtgatca tgaaggccca
     3661 tgttcagtag ataggttgaa aggtgattgt tctgcagaac ttggaggtga tcccagtagt
     3721 aactctgaga aaccacactg tcatcctaaa aaggatgaat gtagttctga aacagatttg
     3781 gaatcatctt gtgaagaaac agaaagtaaa acatctgaca tttcatcacc aataggcagc
     3841 catagagaag aacaagaagg aagagagggc agaggtagca ggcgaactgt tgctaaagga
     3901 aatctgtgtt atattttgaa taaataccac aaaccattcc attgtattca taaaacttgc
     3961 aactcctcat tcaccaatct aaaaggctta attcgccatt acagaactgt acatcagtac
     4021 aacaaagaac agttatgttt ggagaaagac aaagcaagaa ccaaaaggga acttgtcaaa
     4081 tgtaaaaaga tatttgcttg caaatataag gaatgtaata aacgcttcct gtgttccaaa
     4141 gctcttgcta agcactgtag tgattctcat aacctagacc atattgaaga gcctaaagta
     4201 ctttccgaag ctggatctgc agcaaggttt tcttgtaacc agcctcagtg ccctgctgtt
     4261 ttttatacat tcaacaagtt gaagcaccac ttgatggaac agcataatat tgaaggggaa
     4321 atacattcag attatgaaat tcattgtgat cttaatggct gtggccagat tttcacccat
     4381 cgcagtaatt actcacaaca tgtatattac cgacataaag actattatga tgatttgttt
     4441 agaagccaga aagtagcaaa tgagagacta ctaaggagtg aaaaggtatg tcaaacagct
     4501 gatactcagg ggcatgaaca tcagaccacc aggagatcat ttaatgctaa gtctaaaaaa
     4561 tgtggcttaa tcaaagaaaa gaaagcccca ataagtttta aaaccagagc tgaggccctc
     4621 catatgtgtg tggagcactc tgagcacaca cagtacccct gcatggttca aggatgctta
     4681 tctgtggtga agttggagag cagcattgtg aggcattaca aacgcactca tcagatgagt
     4741 agtgcctatt tagagcaaca gatggagaat cttgttgttt gcgttaagta cggtaccaaa
     4801 attaaggagg aacccccttc tgaagcagat ccctgtataa agaaagaaga aaatagaagc
     4861 tgtgaatcag agcgcacaga acacagccat tccccgggtg acagtagtgc acccatccag
     4921 aacactgatt gctgtcattc aagtgaaagg gatggaggtc agaaagggtg catagaaagc
     4981 agctcagtat ttgatgcaga tactctgctc tacaggggaa ctttgaaatg taatcatagt
     5041 tccaaaacca cttccctaga acagtgtaat atagttcagc ctcctcctcc ttgtaaaata
     5101 gaaaattcca tacctaatcc caatgggact gaaagtggga cttatttcac aagtttccag
     5161 ctgcctttac caaggatcaa agaatcagaa actaggcagc atagttcagg gcaagaaaac
     5221 actgtaaaaa atccaaccca tgtcccaaaa gagaatttta ggaaacattc acagccccgg
     5281 tcatttgatt tgaagactta caaacctatg ggatttgaat cttcatttct gaaatttatt
     5341 caggaaagtg aagagaaaga agatgatttt gatgattggg agccttcaga gcacttaaca
     5401 ttaagtaatt cttcacagtc cagtaatgat ttaacaggga atgttgtggc aaataatatg
     5461 gtgaatgaca gtgaacctga agttgacata cctcattctt ccagtgactc tacaattcat
     5521 gagaacctga ctgcaatccc acctttaata gtagctgaaa caacaacagt tccttccttg
     5581 gaaaacctga gggttgtatt ggacaaagca ttaacagact gtggagagct tgccttaaaa
     5641 cagcttcatt atcttcggcc agtggtggtt cttgaaagat ctaagttttc cacaccaatt
     5701 ttagacttat ttccaacaaa aaagacagat gagctttgtg taggaagttc atagtaatga
     5761 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     5821 gagcacgggc cgtttaaagg atacca
//
