LOCUS       sequence-27871-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Kondo K, Kim WY, Lechpammer M, Kaelin WG
  TITLE     Inhibition of HIF2alpha is sufficient to suppress pVHL-defective 
            tumor growth.
  JOURNAL   PLoS Biol. 2003 Dec . 1(3):E83.
  PUBMED    14691554
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2003 Dec . 1(3):E83."
COMMENT     Sequence Label: pRS2 (HIF2a-pRetro-Super)
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(47..63)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(48..64)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(82..498)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(98..114)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(130..152)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tttcaagaga accctttgca gaccttgtct ttttggaaaa gcttatcgat accgtcgacc
       61 tcgagagatc gatctaattc cggcgcctag agaaggagtg agggctggat aaagggagga
      121 tcgaggcggg gtcgaacgag gaggttcaag ggggagagac ggggcggatg gaggaagagg
      181 aggcggaggc ttagggtgta caaagggctt gacccaggga ggggggtcaa aagccaaggc
      241 ttcccaggtc acgatgtagg ggacctggtc tgggtgtcca tgcgggccag gtgaaaagac
      301 cttgatctta acctgggtga tgaggtctcg gttaaaggtg ccgtctcgcg gccatccgac
      361 gttaaaggtt ggccattctg cagagcagaa ggtaacccaa cgtctcttct tgacatctac
      421 cgactggttg tgagcgatcc gctcgacatc tttccagtga cctaaggtca aacttaaggg
      481 agtggtaaca gtctggccct aattttcaga caaatacaga aacacagtca gacagagaca
      541 acacagaacg atgctgcagc gctgcagcag acaagacgcg cggcttcggt tccaaaccga
      601 aagcaaaaat tcagacggag gcgggaactg ttttaggttc tcgtctccta ccagaaccac
      661 atatcctgac ggggtcggat tccacatcga ctcccttcct caggtcgggc cacaaaaacg
      721 gcccccaaag tccctgggac gtctcccagg gttgcggccg ggtgttcaga actcgtcagt
      781 tccaccacgg gtccgccaga tacagagcta gttagctaac tagtacagac gcaggcgcaa
      841 acattagatg ccggcacaaa cacgcac
//
