LOCUS       sequence-27892-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Shyu YJ, Liu H, Deng X, Hu CD
  TITLE     Identification of new fluorescent protein fragments for bimolecular 
            fluorescence complementation analysis under physiological 
            conditions.
  JOURNAL   Biotechniques. 2006 Jan . 40(1):61-6.
  PUBMED    16454041
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2006 Jan . 40(1):61-6."
COMMENT     Sequence Label: pBiFC-CC155
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          124..220
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             284..310
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     601..622
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
       61 atccagcctc cggactctag aggatccggt actagaggaa ctgaaaaacc agaaagttaa
      121 ctggtaagtt tagtcttttt gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat
      181 caaagaactg ctcctcagtg gatgttgcct ttacttctag gcctgtacgg aagtgttact
      241 tctgctctaa aagctgcgga attgtacccg cgggcccacc atgtacccat acgatgttcc
      301 agattacgct cttatggcca tggaggcccg aattcggtcg accgagatct ctcgaggtac
      361 ccgtccggcg tgcaaaatcc cgaacgacct gaaacagaaa gtcatgaacc acgacaagca
      421 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      481 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      541 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      601 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acganctgta
      661 caagtaagcg gccgcgggga tccagacatg ataagataca ttgatgagtt tgganaaacn
      721 ncaactagaa ngcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tanngcttta
      781 tttgtaacca ttataagctg c
//
