LOCUS       sequence-279011-        2300 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1928
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgcggtc ggggaataag gcagctgttg tgctgtgtat ggacgtgggc
       61 tttaccatga gtaactccat tcctggtata gaatccccat ttgaacaagc aaagaaggtg
      121 ataaccatgt ttgtacagcg acaggtgttt gctgagaaca aggatgagat tgctttagtc
      181 ctgtttggta cagatggcac tgacaatccc ctttctggtg gggatcagta tcagaacatc
      241 acagtgcaca gacatctgat gctaccagat tttgatttgc tggaggacat tgaaagcaaa
      301 atccaaccag gttctcaaca ggctgacttc ctggatgcac taatcgtgag catggatgtg
      361 attcaacatg aaacaatagg aaagaagttt gagaagaggc atattgaaat attcactgac
      421 ctcagcagcc gattcagcaa aagtcagctg gatattataa ttcatagctt gaagaaatgt
      481 gacatctccc tgcaattctt cttgcctttc tcacttggca aggaagatgg aagtggggac
      541 agaggagatg gcccctttcg cttaggtggc catgggcctt cctttccact aaaaggaatt
      601 accgaacagc aaaaagaagg tcttgagata gtgaaaatgg tgatgatatc tttagaaggt
      661 gaagatgggt tggatgaaat ttattcattc agtgagagtc tgagaaaact gtgcgtcttc
      721 aagaaaattg agaggcattc cattcactgg ccctgccgac tgaccattgg ctccaatttg
      781 tctataagga ttgcagccta taaatcgatt ctacaggaga gagttaaaaa gacttggaca
      841 gttgtggatg caaaaaccct aaaaaaagaa gatatacaaa aagaaacagt ttattgctta
      901 aatgatgatg atgaaactga agttttaaaa gaggatatta ttcaagggtt ccgctatgga
      961 agtgatatag ttcctttctc taaagtggat gaggaacaaa tgaaatataa atcggagggg
     1021 aagtgcttct ctgttttggg attttgtaaa tcttctcagg ttcagagaag attcttcatg
     1081 ggaaatcaag ttctaaaggt ctttgcagca agagatgatg aggcagctgc agttgcactt
     1141 tcctccctga ttcatgcttt ggatgactta gacatggtgg ccatagttcg atatgcttat
     1201 gacaaaagag ctaatcctca agtcggcgtg gcttttcctc atatcaagca taactatgag
     1261 tgtttagtgt atgtgcagct gcctttcatg gaagacttgc ggcaatacat gttttcatcc
     1321 ttgaaaaaca gtaagaaata tgctcccacc gaggcacagt tgaatgctgt tgatgctttg
     1381 attgactcca tgagcttggc aaagaaagat gagaagacag acacccttga agacttgttt
     1441 ccaaccacca aaatcccaaa tcctcgattt cagagattat ttcagtgtct gctgcacaga
     1501 gctttacatc cccgggagcc tctaccccca attcagcagc atatttggaa tatgctgaat
     1561 cctcccgctg aggtgacaac aaaaagtcag attcctctct ctaaaataaa gacccttttt
     1621 cctctgattg aagccaagaa aaaggatcaa gtgactgctc aggaaatttt ccaagacaac
     1681 catgaagatg gacctacagc taaaaaatta aagactgagc aagggggagc ccacttcagc
     1741 gtctccagtc tggctgaagg cagtgtcacc tctgttggaa gtgtgaatcc tgctgaaaac
     1801 ttccgtgttc tagtgaaaca gaagaaggcc agctttgagg aagcgagtaa ccagctcata
     1861 aatcacatcg aacagttttt ggatactaat gaaacaccgt attttatgaa gagcatagac
     1921 tgcatccgag ccttccggga agaagccatt aagttttcag aagagcagcg ctttaacaac
     1981 ttcctgaaag cccttcaaga gaaagtggaa attaaacaat taaatcattt ctgggaaatt
     2041 gttgtccagg atggaattac tctgatcacc aaagaggaag cctctggaag ttctgtcaca
     2101 gctgaggaag ccaaaaagtt tctggccccc aaagacaaac caagtggaga cacagcagct
     2161 gtatttgaag aaggtggtga tgtggacgat ttattggaca tgatatagta atgaactagt
     2221 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgacggg
     2281 tatgtccata tggcgcctga
//
