LOCUS       sequence-279023-        1769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1950
FEATURES             Location/Qualifiers
     source          1..1769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggaggc ttcttttgga agttcgagcc cagtttgttt tattccagtt
       61 gggtctttgt cttctgagga tcatgatttt gaccccactg ctgagatgtt ggtccatgac
      121 tatgatgatg aaagaactct tgaagaagag gaaatgatgg atgagggtaa aaacttcagt
      181 tcagaaattg aagacttaga aaaggaagga accatgcctc tagaagattt actggcattc
      241 tatggctatg aacctacaat tccagcagtt gcaaattcca gtgcaaatag ttccccaagt
      301 gaactggcag atgaactacc agacatgaca ctagacaaag aggaaatagc aaaagacctg
      361 ttgtcaggtg atgacgagga aactcagtct tctgcggatg atctgacgcc atctgtgact
      421 tcccatgaaa cttctgattt cttccctagg cctttacgat caaatactgc atgtgatggt
      481 gataaggaat cagaggttga agatgttgaa acagacagtg gtaattcacc tgaagatttg
      541 aggaaggaaa taatgattgg tttacaatat caggcagaga ttccccctta tcttggagag
      601 tacgatggta atgagaaagt atatgaaaac gaagaccagt tactttggtg tcctgatgtg
      661 gttttggaga gcaaagttaa ggaatacctt gttgagactt cattaaggac tggcagtgaa
      721 aaaataatgg ataggatttc tgcaggaaca cacacaaggg acaatgaaca ggcattatat
      781 gaacttctca agtgtaacca caatataaag gaagcaatcg aaagatactg ctgcaatgga
      841 aaggcctctc aagaaggaat gactgcatgg acggaagaag aatgccgaag ctttgaacat
      901 gcactcatgc tttttggaaa agattttcat cttatacaga agaataaggt gagaactagg
      961 acagttgctg agtgtgtagc attctactat atgtggaaga aatctgaacg ttatgattac
     1021 tttgctcaac agacaagatt tgggaaaaaa agatataacc atcaccctgg agttacggac
     1081 tatatggatc gtttagtaga tgaaacagaa gctttgggtg ggacggtaaa tgcttcagcc
     1141 ttaacttcta accggcctga gcctattcct gatcaacagc taaacattct caactccttc
     1201 actgccagtg acttgacagc tttgaccaac agtgtagcaa ccgtctgcga ccccacagat
     1261 gtgaattgtt tggatgatag ctttcctcca ctgggcaaca caccccgtgg acaagttaat
     1321 catgtgcctg ttgtaacaga agagttactc accctgccca gcaatgggga aagtgattgt
     1381 tttaatttat ttgagactgg attttatcac tcggagctaa accctatgaa catgtgcagt
     1441 gaagagtcag agagaccagc aaaaagattg aaaatgggca ttgccgtccc tgaatccttt
     1501 atgaatgaag tttctgtaaa taacctgggt gtggactttg aaaatcacac acatcacatc
     1561 accagtgcca aaatggctgt ttctgtggct gactttggca gtctctctgc caacgagacc
     1621 aatggtttca tcagtgccca tgctctgcat cagcacgcgg ccctacactc tgagtagtaa
     1681 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1741 gacgataatg gagcgtcgca tagggtttg
//
