LOCUS       sequence-279137-        1919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2589
FEATURES             Location/Qualifiers
     source          1..1919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgctgg gcagcgggaa gcgaggcccc aggtgtcact gacgtttgag
       61 gatgtggctg tgctgtttac ccgagatgag tggagaaagc tggccccttc tcagagaaac
      121 ttgtaccggg atgtgatgct ggagaactat aggaacctgg tctcactggg gctcccattt
      181 accaaaccaa aagtgatctc cctgttgcag caaggagaag atccctggga ggtggagaaa
      241 gacggttctg gcgtctcctc tctaggatcg aagagcagtc ataaaaccac aaagtcaacg
      301 caaacacaag actcttcatt tcagggactg atactgaaaa gatccaacag gaatgtacct
      361 tgggatttga aattagaaaa gccttacata tatgaaggca gattagagaa aaagcaggat
      421 aaaaagggaa gttttcagat agtttcagcc acccacaaaa aaatccccac tatagaaaga
      481 agccataaaa atactgaatt gagccaaaac ttcagcccaa agtcagtgct tattaggcaa
      541 cagatacttc ccagagaaaa aacaccacca aaatgtgaaa tacaaggaaa cagcctcaaa
      601 cagaattcac aattacttaa tcaaccaaaa attacagcag ataaacgcta taaatgtagt
      661 ctgtgtgaaa aaaccttcat taacacttca tcccttcgta aacatgagaa aaaccatagt
      721 ggagagaaac tatttaagtg taaagaatgt tcaaaagcct ttagccaaag ttcagctctt
      781 attcaacatc aaataacgca tactggagag aaaccctaca tatgtaaaga atgtgggaaa
      841 gcctttactc tcagtacatc cctttataaa catctaagaa cccatactgt ggagaaatcc
      901 tacagatgta aagaatgtgg taaatccttc agccgaaggt caggcctttt tatacatcaa
      961 aaaattcatg ctgaagaaaa cccttgtaag tataatccgg gtaggaaggc atctagttgc
     1021 agcacatccc tttctggatg tcaaagaatt cattctagaa agaagtccta cttatgtaat
     1081 gaatgtggca acacctttaa gtctagctca tcccttcgtt atcatcagag aattcacact
     1141 ggagagaagc cttttaaatg tagtgaatgt gggagagcct tcagccagag tgcctctctt
     1201 attcaacatg aaagaattca caccggagaa aagccctata gatgcaatga atgtgggaaa
     1261 ggctttactt ctatttcacg acttaataga caccgaatca ttcatactgg agagaagttt
     1321 tataattgta atgaatgtgg taaagcctta agctcccact caacacttat tattcacgag
     1381 cgaattcata ctggagaaaa accatgtaaa tgtaaagtat gtggaaaagc cttcagacag
     1441 agttcagctc tcattcaaca tcagagaatg catactggag aaagacccta taaatgtaac
     1501 gagtgtggga aaacattcag gtgtaactca tcacttagta atcaccagag aattcatact
     1561 ggagagaaac catatcgatg tgaggaatgt gggatatctt ttggccaaag ttcagctctt
     1621 attcagcatc gaaggattca tacaggagaa aaacccttta aatgtaatac atgtggaaaa
     1681 acttttagac aaagctcatc acgtattgca catcagagaa ttcatactgg agagaaaccc
     1741 tatgaatgta atacatgtgg gaaacttttc aaccataggt catcccttac taatcattat
     1801 aaaattcata tcgaagagga cccctagtaa tgaactagtc cgtaacttga aagtatttcg
     1861 atttcttggc tttatatatc ttgtggaaag gacgaggcat ccaatgacat atacagatt
//
