LOCUS       sequence-27926-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Todi SV, Laco MN, Winborn BJ, Travis SM, Wen HM, Paulson HL
  TITLE     Cellular turnover of the polyglutamine disease protein ataxin-3 is 
            regulated by its catalytic activity.
  JOURNAL   J Biol Chem. 2007 Oct 5. 282(40):29348-58.
  PUBMED    17693639
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Oct 5. 282(40):29348-58."
COMMENT     Sequence Label: pVETL-Cmcs-Flag-Ataxin3Q80
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(550..573)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(654..678)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(675..726)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(704..724)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 canngcggnt ggtngcttcg catctggcag atcaccctta acaacaaata tagaataacc
       61 ttcctgttgt aattgagcca agaaaagtgc aagatatgta tctgatatta attctggacc
      121 cgtcaagaga gaattcaagt taaaccactg ttttcctaat tttctaactg taaaccagtg
      181 ttccttataa ttgcatataa atgatctttc atttatagga tcgatcctga gcctctgata
      241 ctctggactg ttgaacagga ttagttctaa accccaaact ttcaaggcat tgcttataac
      301 ctgaatagag aaaaaaccac tgtcatccat atttccagaa ggctgctgta aaaacgtgcg
      361 ataatcttca ctagtaactc ctccttctgc cattctcatc ctctcctcct catccagctg
      421 atgtgcaatt gaggataatt ccacagggct aaaatattct ccttgcaata agttattcag
      481 gcaatgttga gcacaaagtg agccttcttg tttctcgtgg aagatggact ctccgtttat
      541 aagcttgagc ttgtcgtcat cgtctttgta gtccatagct ctagagtccg gaggctggat
      601 cggtcccggt gtcttctatg gaggtcaaaa cagcgtggat ggcgtctccn ggcgatctga
      661 cggttcacta aacgagctct gcttatatag acctcncacc gtacacgcct accgcccatt
      721 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt tggtgccaac
      781 aactccatga cgtcatgggt ggnactggan ccccggagtc aacgcttccc nccctngtgt
      841 ggcanccgc
//
