LOCUS       sequence-279284-        1151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1365
FEATURES             Location/Qualifiers
     source          1..1151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      800..809
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggagacat gggagatcca ccaaaaaaaa aacgtctgat ttccctatgt
       61 gttggttgcg gcaatcagat tcacgatcag tatattctga gggtttctcc ggatttggaa
      121 tggcatgcgg catgtttgaa atgtgcggag tgtaatcagt atttggacga gagctgtaca
      181 tgctttgtta gggatgggaa aacctactgt aaaagagatt atatcaggtt gtacgggatc
      241 aaatgcgcca agtgcagcat cggcttcagc aagaacgact tcgtgatgcg tgcccgctcc
      301 aaggtgtatc acatcgagtg tttccgctgt gtggcctgca gccgccagct catccctggg
      361 gacgaatttg cgcttcggga ggacggtctc ttctgccgag cagaccacga tgtggtggag
      421 agggccagtc taggcgctgg cgacccgctc agtcccctgc atccagcgcg gccactgcaa
      481 atggcagcgg agcccatctc cgccaggcag ccagccctgc ggccccacgt ccacaagcag
      541 ccggagaaga ccacccgcgt gcggactgtg ctgaacgaga agcagctgca caccttgcgg
      601 acctgctacg ccgcaaaccc gcggccagat gcgctcatga aggagcaact ggtagagatg
      661 acgggcctca gtccccgtgt gatccgggtc tggtttcaaa acaagcggtg caaggacaag
      721 aagcgaagca tcatgatgaa gcaactccag cagcagcagc ccaatgacaa aactaatatc
      781 caggggatga caggaactcc catggtggct gccagtccag agagacacga cggtggctta
      841 caggctaacc cagtggaagt acaaagttac cagccacctt ggaaagtact gagcgacttc
      901 gccttgcaga gtgacataga tcagcctgct tttcagcaac tggtcaattt ttcagaagga
      961 ggaccgggct ctaattccac tggcagtgaa gtagcatcaa tgtcctctca acttccagat
     1021 acacctaaca gcatggtagc cagtcctatt gaggcatagt aatgaactag tccgtaactt
     1081 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaag taacggctcg
     1141 tgggaagtgt c
//
