LOCUS       sequence-279374-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2385
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcggaa gaggtgggag tgcccggcgc tcccgcaggg ctgggagagg
       61 gaagaagtgc ccagaaggtc ggggctgtcg gccggccaca gggatgtctt ttactatagc
      121 ccgagcggga agaagttccg cagcaagccg cagctggcgc gctacctggg cggctccatg
      181 gacctgagca ccttcgactt ccgcacgggc aagatgctga tgagcaagat gaacaagagc
      241 cgccagcgcg tgcgctacga ctcctccaac caggtcaagg gcaagcccga cctgaacacg
      301 gcgctgcccg tgcgccagac ggcgtccatc ttcaagcagc cggtgaccaa gattaccaac
      361 caccccagca acaaggtcaa gagcgacccg cagaaggcgg tggaccagcc gcgccagctc
      421 ttctgggaga agaagctgag cggcctgaac gccttcgaca ttgctgagga gctggtcaag
      481 accatggacc tccccaaggg cctgcagggg gtgggacctg gctgcacgga tgagacgctg
      541 ctgtcggcca tcgccagcgc cctgcacact agcaccatgc ccatcacggg acagctctcg
      601 gccgccgtgg agaagaaccc cggcgtatgg ctcaacacca cgcagcccct gtgcaaagcc
      661 ttcatggtga ccgacgagga catcaggaag caggaagagc tggtgcagca ggtgcggaag
      721 cggctggagg aggcgctgat ggccgacatg ctggcgcacg tggaggagct ggcccgtgac
      781 ggggaggcgc cgctggacaa ggcctgcgct gaggacgacg acgaggaaga cgaggaggag
      841 gaggaggagg agcccgaccc ggacccggag atggagcacg tctagtaatg aactagtccg
      901 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgacgcgttc
      961 ccgtgaagga aagagga
//
