LOCUS       sequence-279407-         578 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2434
FEATURES             Location/Qualifiers
     source          1..578
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggcgacaa cccttttcaa ccaaaaagta attcaaaaat ggcagaactg
       61 tttatggaat gtgaagaaga ggagctggaa ccatggcaga agaaagtaaa agaagttgag
      121 gatgacgatg atgatgagcc aatctttgtt ggcgagatat caagttcaaa accagcaatt
      181 tcaaatattt tgaacagagt taaccccagc tcatattcaa ggggactaaa gaatggtgca
      241 ctcagtcgag gtattactgc tgcattcaag cctacaagtc aacactacac gaatccaaca
      301 tcaaatccag tgcctgcctc accaataaat tttcatcctg agtctagatc ttcagatagt
      361 tctgttattg ttcagccttt ttctaaacct gtaagtgttt ctaaaactat acggccagct
      421 cagggatcca ttggatgttg tttatcaata tcaacagtac ccagttacaa ttctggactg
      481 tcatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      541 tgtggaaagg acgaaatttg ttattggacc cgacgctt
//
