LOCUS       sequence-279773-        1835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0149
FEATURES             Location/Qualifiers
     source          1..1835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      551..560
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgatctg ctgtgcggcc gtgaactgct ccaaccggca gggaaagggc
       61 gagaagcgcg ccgtctcctt ccacaggttc cccctaaagg actcaaaacg tctaatccaa
      121 tggttaaaag ctgttcagag ggataactgg actcccacta agtattcatt tctctgtagt
      181 gagcatttca ccaaagacag cttctccaag aggctggagg accagcatcg cctgctgaag
      241 cccacggccg tgccatccat cttccacctg accgagaaga agaggggggc tggaggccat
      301 ggccgcaccc ggagaaaaga tgccagcaag gccacagggg gtgtgagggg acactcgagt
      361 gccgccacca gcagaggagc tgcaggttgg tcaccgtcct cgagtggaaa cccgatggcc
      421 aagccagagt cccgcaggtt gaagcaagct gctctgcaag gtgaagccac acccagggcg
      481 gcccaggagg ccgccagcca ggagcaggcc cagcaagctc tggaacggac tccaggagat
      541 ggactggcca ccatggtggc aggcagtcag ggaaaagcag aagcgtctgc cacagatgct
      601 ggcgatgaga gcgccacttc ctccatcgaa gggggcgtga cagataagag tggcatttct
      661 atggatgact ttacgccccc aggatctggg gcgtgcaaat ttatcggctc acttcattcg
      721 tacagtttct cctctaagca cacccgagaa aggccatctg tcccccgaga gcccattgac
      781 cgcaagaggc tgaagaaaga tgtggaacca agctgcagtg ggagcagcct gggacccgac
      841 aagggcctgg cccagagccc tcccagctca tcacttaccg cgacaccgca gaagccttcc
      901 cagagcccct ctgcccctcc tgccgacgtc accccaaagc cagccacgga agccgtgcag
      961 agcgagcaca gcgacgccag ccccatgtcc atcaacgagg tcatcctgtc ggcgtcaggg
     1021 gcctgcaagc tcatcgactc actgcactcc tactgcttct cctcccggca gaacaagagc
     1081 caggtgtgct gcctgcggga gcaggtggag aagaagaacg gcgagctgaa gagcctgcgg
     1141 cagagggtca gccgctccga cagccaggtg cggaagctac aggagaagct ggatgagctg
     1201 aggagagtga gcgtccccta tccaagtagc ctgctgtcgc ccagccgcga gccccccaag
     1261 atgaacccag tggtggagcc actgtcctgg atgctgggca cctggctgtc ggacccacct
     1321 ggagccggga cctaccccac actgcagccc ttccagtacc tggaggaggt tcacatctcc
     1381 cacgtgggcc agcccatgct gaacttctcg ttcaactcct tccacccgga cacgcgcaag
     1441 ccgatgcaca gagagtgtgg cttcattcgc ctcaagcccg acaccaacaa ggtggccttt
     1501 gtcagcgccc agaacacagg cgtggtggaa gtggaggagg gcgaggtgaa cgggcaggag
     1561 ctgtgcatcg catcccactc catcgccagg atctccttcg ccaaggagcc ccacgtagag
     1621 cagatcaccc ggaagttcag gctgaattct gaaggcaaac ttgagcagac ggtctccatg
     1681 gcaaccacga cacagccaat gactcagcat cttcacgtca cctacaagaa ggtgaccccg
     1741 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1801 ggaaaggacg atgaccacta tcgtcaagat ccatc
//
