LOCUS       sequence-279989-        1985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0413
FEATURES             Location/Qualifiers
     source          1..1985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagccac tgagatctct gtcctttctg agcaattcac caagatcaaa
       61 gaactcgagt tgatgccgga aaaaggcctg aaggaggagg aaaaagacgg agtgtgcaga
      121 gagaaagacc atcggagccc tagtgagttg gaggccgagc gtacctctgg ggccttccag
      181 gacagcgtcc tggaggaaga agtggagctg gtgctggccc cctcggagga gagcgagaag
      241 tacatcctga ccctgcagac ggtgcacttc acttctgaag ctgtggagtt gcaggatatg
      301 agcttgctga gcatacagca gcaagaaggg gtgcaggtgg tggtgcaaca gcctggccct
      361 gggttgctgt ggcttgagga agggccccgg cagagcctgc agcagtgtgt ggccattagt
      421 atccagcaag agctgtactc cccgcaagag atggaggtgt tgcagttcca cgctctagag
      481 gagaatgtga tggtggccag tgaagacagt aagttagcgg tgagcctggc tgaaactact
      541 ggactgatca agctcgagga agagcaggag aagaaccagt tattggctga aagaacaaag
      601 gagcagctct tttttgtgga aacaatgtca ggagatgaaa gaagtgacga aattgttctc
      661 acagtttcaa attcaaatgt ggaagaacaa gaggatcaac ctacagctgg tcaagcagat
      721 gctgaaaagg ccaaatctac aaaaaatcaa agaaagacaa agggagcaaa aggaaccttc
      781 cactgtgatg tctgcatgtt cacctcttct agaatgtcaa gttttaatcg tcatatgaaa
      841 actcacacca gtgagaagcc tcacctgtgt cacctctgcc tgaaaacctt ccgtacggtc
      901 actctgctgc ggaaccatgt taacacccac acaggaacca ggccctacaa gtgtaacgac
      961 tgcaacatgg catttgtcac cagtggagaa ctcgtccgac acaggcgcta taaacatact
     1021 catgagaaac cctttaaatg ttccatgtgc aagtatgcca gtgtggaggc aagtaaattg
     1081 aagcgccatg tccgatccca cactggggag cgcccctttc agtgttgcca gtgcagctat
     1141 gccagcagag atacctacaa gctgaaacgc cacatgagaa cgcactcagg tgagaagcct
     1201 tacgaatgcc acatctgcca cacccgcttc acccagagcg ggaccatgaa aatacatatt
     1261 ctgcagaaac acggcgaaaa tgtccccaaa taccagtgtc cccattgtgc caccatcatt
     1321 gcacggaaaa gcgacctacg tgtgcatatg cgcaacttgc atgcttacag cgctgcagag
     1381 ctgaaatgcc gctactgttc tgctgtcttc catgaacgct atgccctcat tcagcaccag
     1441 aaaactcata agaatgagaa gaggttcaag tgcaaacact gcagttatgc ctgcaagcag
     1501 gaacgtcata tgaccgctca cattcgtacc cacactggag agaaaccatt cacctgcctt
     1561 tcttgcaata aatgtttccg acagaagcaa cttctaaacg ctcacttcag gaaataccac
     1621 gatgcaaatt tcatcccgac tgtttacaaa tgctccaagt gtggcaaagg cttttcccgc
     1681 tggattctct gggttgggaa ctcggaagtg gctgaactgg gtggtcctgg ctcagggcca
     1741 ctcctgaggc tgcagtcagg atgtccgcca gggctgcatc atccgaaggc tggactgggg
     1801 ccagaggatc cacttccagg acagctccgc cacacaactg ctggcaccgg cctcagttcc
     1861 ttgctacagg gacctctctg cagggctgct tagtaatgaa ctagtccgta acttgaaagt
     1921 atttcgattt cttggcttta tatatcttgt ggaaaggacg accatctgtc agggtaaccc
     1981 agttg
//
