LOCUS       sequence-280001-        1241 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0430
FEATURES             Location/Qualifiers
     source          1..1241
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaatctga gaaaatttat atgaccgcga acccatatct ctgtactgaa
       61 tgtgggaaag gctacacttg tcttgcatcc ctgacccagc atcagaaaac acatattgga
      121 gaaaaacctt atgaatgtaa aatatgtggt aagtccttta cccggaacag taatcttgtt
      181 caacatcaaa gaatacatac tggagagaaa ccttatgaat gtaatgaatg tgggaaagct
      241 tttagtcaga gcactaatct tattcagcat caaagagtcc atactgggga gaaaccttat
      301 gagtgtaatg aatgtgaaaa aacctttagt cataggtcat cccttagaaa tcatgagaga
      361 atccatactg gagaaaaacc ctatccttgt aatgaatgtg ggaaagcttt cagccatatc
      421 tctgccctta ctcaacatca tagaattcat actggaaaga aaccatatga atgtactgaa
      481 tgtggaaaaa ccttcagccg cagcacacac ctaattgaac atcagggaat tcattctgag
      541 gaaaaatcct accagtgtaa gcaatgtcgg aaagtttttt gccacagtac atcactaatc
      601 cgacatcaga gaacccacac aggagagaaa ccatatgaat gtaatgaatg tgggaaagct
      661 ttcagccata ccccagcctt cattcaacat caaagaattc atactggaga aaaaccctat
      721 gagtgtaatg catgtggaaa ggccttcaat cggagtgcac atcttactga acaccagaga
      781 actcatactg gagagaagcc ctatgtttgt aaagaatgtg gaaaaacctt cagtcgaagt
      841 acacacctta ctgaacatct aaaaattcat tcttgtgtga aaccctatca atgtaatgaa
      901 tgtcagaaac tgttttgcta tagaacatca ctaattcgac atcagaggac gcatacagga
      961 gagaaaccct accagtgtaa tgaatgtggg aaatccttca gcttaagctc agctctaact
     1021 aaacataagc gaatacatac aagggagaga ccctatcaat gtactaagtg tggtgatgtc
     1081 ttttgtcata gtacatcctt aattcgacat cagaaaactc atttcagaaa ggaaacctta
     1141 gcagagtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1201 tcttgtggaa aggacgaaac aacctcgcca accgaaatat g
//
