LOCUS       sequence-280109-        1694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0604
FEATURES             Location/Qualifiers
     source          1..1694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgacggc gaccatagct ctcggcacag actccatcaa gatggagaac
       61 gggcagagca cagccgccaa gctggggctg cctcccctga cgcccgagca gcaggaggcc
      121 cttcagaagg ccaagaagta cgccatggag cagagcatca agagtgtgct ggtgaagcag
      181 accatcgcgc accagcagca gcagctcacc aacctgcaga tggcagcagt gacaatgggc
      241 tttggagatc ctctctcacc tttgcaatcg atggcggctc agcggcagcg ggcgctggcc
      301 atcatgtgcc gcgtctacgt gggctctatc tactatgagc tgggggagga caccatccgc
      361 caggcctttg ccccctttgg ccccatcaag agcatcgaca tgtcctggga ctccgtcacc
      421 atgaagcaca agggctttgc cttcgtggag tatgaggtcc ccgaagctgc acagctggcc
      481 ttggagcaga tgaactcggt gatgctgggg ggcaggaaca tcaaggtggg cagacccagc
      541 aacatagggc aggcccagcc catcatagac cagttggctg aggaggcacg ggccttcaac
      601 cgcatctacg tggcctctgt gcaccaggac ctctcagacg atgacatcaa gagcgtgttt
      661 gaggcctttg gcaagatcaa gtcctgcaca ctggcccggg accccacaac tggcaagcac
      721 aagggctacg gcttcattga gtacgagaag gcccagtcgt cccaagatgc tgtgtcttcc
      781 atgaacctct ttgacctggg tggccagtac ttgcgggtgg gcaaggctgt cacaccgccc
      841 atgcccctac tcacaccagc cacgcctgga ggcctcccac ctgccgctgc tgtggcagct
      901 gctgcagcca ctgccaagat cacagctcag gaagcagtgg ccggagcagc ggtgctgggt
      961 accctgggca cacctggact ggtgtcccca gcactgaccc tggcccagcc cctgggcact
     1021 ttgccccagg ctgtcatggc tgcccaggca cctggagtca tcacaggtgt gaccccagcc
     1081 cgtcctccta tcccggtcac catcccctcg gtgggagtgg tgaaccccat cctggccagc
     1141 cctccaacgc tgggtctcct ggagcccaag aaggagaagg aagaagagga gctgtttccc
     1201 gagtcagagc ggccagagat gctgagcgag caggagcaca tgagcatctc gggcagtagc
     1261 gcccgacaca tggtgatgca gaagctgctc cgcaagcagg agtctacagt gatggttctg
     1321 cgcaacatgg tggaccccaa ggacatcgat gatgacctgg aaggggaggt gacagaggag
     1381 tgtggcaagt tcggggccgt gaaccgcgtc atcatctacc aagagaaaca aggcgaggag
     1441 gaggatgcag aaatcattgt caagatcttt gtggagtttt ccatagcctc tgagactcat
     1501 aaggccatcc aggccctcaa tggccgctgg tttgctggcc gcaaggtggt ggctgaagtg
     1561 tacgaccagg agcgttttga taacagtgac ctctctgcgt agtaatgaac tagtccgtaa
     1621 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga ggtaacccgc
     1681 ccatagacac ggat
//
