LOCUS       sequence-280130-        1541 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0617
FEATURES             Location/Qualifiers
     source          1..1541
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcaacagc attggtgagt gcccattccc tggctcccct gaatctgaag
       61 aaggaggggc ttcgggtagt gagggaggat cactactcta cttgggaaca gggattcaag
      121 ctgcaaggaa acagtaaagg ccttggacag gagccattgt gcaaacaatt caggcagttg
      181 cgttatgaag agaccacagg acctcgagaa gcactaagtc ggctccggga gctctgtcaa
      241 cagtggctac agcccgagac ccataccaag gagcagatcc tggagctgct ggtgctggag
      301 cagtttctga tcatcctgcc taaggagctc caggcccggg tgcaggagca tcacccagag
      361 agcagggagg acgtggttgt tgttctggag gatttgcagc tggatcttgg agaaacagga
      421 caacaggtgg acccagacca gccaaagaaa caaaaaatac ttgtggagga gatggcccct
      481 ctgaaaggag tacaggaaca gcaggttcgg catgagtgtg aagttacaaa gcctgagaaa
      541 gagaagggtg aggagacaag gattgagaat gggaagctta ttgtagtaac agactcttgt
      601 ggaagagtag agtcatctgg gaaaatatct gaacccatgg aggctcataa tgagggctct
      661 aacttggaaa ggcatcaggc caagcccaaa gagaagattg agtataaatg ctcagaacgt
      721 gagcagagat tcatccagca cttggacctg attgaacatg cgagtacaca cacgggaaag
      781 aaactctgcg agtctgatgt gtgtcagagt tccagtctta caggacataa gaaagtcctc
      841 tctagagaga aaggtcatca gtgtcatgag tgtgggaaag cctttcagag gagttcacac
      901 ctcgtcagac atcagaaaat ccatcttggt gagaagcctt atcagtgcaa tgagtgtggc
      961 aaagtcttta gccagaatgc aggccttttg gaacatctca gaattcatac tggagagaaa
     1021 ccttatctat gtatccattg tggaaaaaat tttaggcgca gctctcacct taatcgacat
     1081 cagagaattc acagtcagga ggagccctgt gagtgcaagg agtgtggaaa aacctttagt
     1141 caggccttac tcctcaccca ccatcagaga atccatagtc actccaaaag ccatcaatgt
     1201 aacgagtgtg gaaaagcttt cagtttgacc tcagacctta ttcgacacca cagaattcat
     1261 actggagaaa aacctttcaa gtgtaacata tgccagaaag ccttccgact aaactcacac
     1321 cttgctcagc atgtaagaat ccacaatgaa gaaaaaccct atcagtgtag tgaatgtgga
     1381 gaagccttca ggcaaaggtc aggtcttttt caacatcaga gatatcacca caaagacaaa
     1441 ctggcttagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1501 tcttgtggaa aggacgacat tgttgtgatt tcgaggaggg t
//
