LOCUS       sequence-280184-        1820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0745
FEATURES             Location/Qualifiers
     source          1..1820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggattt cgaagagttg aggaatatgg tttctagttt tagggtttct
       61 gaactacaag tattactagg ctttgctgga cggaataaaa gtggacgcaa gcatgacctc
      121 ctgatgaggg cgctgcattt attgaagagc ggctgcagcc ctgcggttca gattaaaatc
      181 cgagaattgt atagacgccg atatccacga actcttgaag gactttctga tttatccaca
      241 atcaaatcat cggttttcag tttggatggt ggctcatcac ctgtagaacc tgacttggcc
      301 gtggctggaa tccactcgtt gccttccact tcagttacac ctcactcacc atcctctcct
      361 gttggttctg tgctgcttca agatactaag cccacatttg agatgcagca gccatctccc
      421 ccaattcctc ctgtccatcc tgatgtgcag ttaaaaaatc tgccctttta tgatgtcctt
      481 gatgttctca tcaagcccac gagtttagtt caaagcagta ttcagcgatt tcaagagaag
      541 ttttttattt ttgctttgac acctcaacaa gttagagaga tatgcatatc cagggatttt
      601 ttgccaggtg gtaggagaga ttatacagtc caagttcagt tgagactttg cctggcagag
      661 acaagttgcc ctcaagaaga taactatcca aatagtctat gtataaaagt aaatgggaag
      721 ctatttcctt tgcctggcta tgcaccaccg cctaaaaatg ggattgaaca gaagcgccct
      781 ggacgcccct tgaatattac atctttagtt aggttatctt cagctgtgcc aaaccaaatt
      841 tccatttctt gggcatcaga aattgggaag aattactcta tgtctgtata tcttgtacgg
      901 cagcttacat cagccatgtt attacagaga ttaaaaatga aaggtattag aaaccctgat
      961 cattccagag cactaattaa agaaaaactt actgcagatc ctgatagtga aattgctaca
     1021 actagccttc gggtatcctt gatgtgccct ttaggaaaaa tgaggctgac aatcccatgc
     1081 cgtgcagtga cttgtacaca tctgcagtgt tttgatgctg ccctctatct acaaatgaat
     1141 gagaaaaagc ccacctggat ttgtcctgtg tgtgacaaaa aagctgccta tgaaagtcta
     1201 atattagatg ggctttttat ggaaattctc aatgactgtt ctgatgtaga tgagatcaaa
     1261 ttccaagaag atggttcttg gtgtccaatg agaccgaaga aagaagctat gaaagtatcc
     1321 agccaaccgt gtacaaaaat agaaagttca agcgtcctca gtaagccttg ttcagtgact
     1381 gtagccagtg aggcaagcaa gaagaaagta gatgttattg atcttacaat agaaagctct
     1441 tctgacgaag aggaagaccc tcctgccaaa aggaaatgca tctttatgtc agaaacacaa
     1501 agcagcccaa ccaaaggggt tctcatgtat cagccatctt ctgtaagggt gcccagtgtg
     1561 acttcggttg atcctgctgc tattccgcct tcattaacag actactcagt accattccac
     1621 catacgccaa tatcaagcat gtcatcagat ttgccaggag aacaaagaag aaatgatatt
     1681 aataatgaac tgaagcttgg aacatcttct gatactgtgc aacagtagta atgaactagt
     1741 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgactag
     1801 cgtcagcctg gcttcagttc
//
