LOCUS       sequence-280307-        2177 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1080
FEATURES             Location/Qualifiers
     source          1..2177
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctctcct tttgacacta acatcaccag acctggaggg tacctgggac
       61 accagagaca aggatggctt caaagcccag gaggggccgc ccctggctgt acctgaattt
      121 cctgtgtgtg gactgtaccg gatctacgga gtctgtggct ctttcagctc cttcttcatc
      181 atccggtgca gcctttgtgc tctggagacc ctcaaatcac cccaacatga tcccttagag
      241 atacctgaac agtccctcaa actcataccc ctggtgtctg ggaaaaggga actcacaagg
      301 ggccagaaag ctggagagaa gcccctggct gcaggacccg gggaggagga actgctccgg
      361 ggctcagccc ctcatgctca ggacactcag agtgaggaac tgccaccctc ctgcaccatc
      421 tcaggagaga agaagccgcc agcagtctct ggagaagcca ccggggctga tgctgggaga
      481 ctgtgcccgc ccccccgctc cagggctccc cacaaagaca gaactctagc ccgctccagg
      541 ccccagactc agggggaaga ttgttccctc ccagtgggag aggtgaagat aggaaagagg
      601 tcctattctc cagcccccgg gaagcagaaa aagcctaatg ccatgggtct ggccccaaca
      661 tcatctccgg gtgcccctaa ctcagcccgt gccacacaca acccagtgcc ctgtgggtca
      721 ggccgggggc cctgccacct ggccaatctc ctcagtacat tggcgcagag caaccaaaac
      781 agagaccaca agcaggggcc cccggaagtg acctgccaaa ttaggaaaaa gacacgaacc
      841 ctataccgct cagatcagct ggaggagcta gagaagatat tccaagaaga ccactatcct
      901 gacagtgata aacgccgaga gattgcccag acggtggggg tgacccccca gcgcatcatg
      961 gtaaaggggg ccggctcact ggtggcaggg tggagtggcg gagggcccac cattgaaaca
     1021 ctcgaattgc agagtgagcg ctcagcggta gcctgggtgt ggttccagaa tcgccgggcc
     1081 aagtggcgaa aaatggagaa actgaatggg aaagaaagca aggacaatcc tgcagcccct
     1141 ggccctgcca gcagtcaatg cagctctgca gctgagatcc tacctgctgt gcccatggag
     1201 ccaaagcctg accctttccc tcaggagtcc cctctggata cctttccaga gccccccatg
     1261 ctgctgactt ctgaccagac tttggccccc acccaaccca gtgagggtgc tcagagggtg
     1321 gtgacccccc cactcttcag ccccccacct gtgcgaaggg ccgatcttcc tttccccctt
     1381 ggccctgtcc acacccccca actgatgcca ctgctgatgg atgttgctgg cagtgacagc
     1441 agccacaagg acggcccctg tgggtcctgg gggacaagca tcaccctgcc acccccctgt
     1501 tcatatttgg aggagctgga gccccaggat taccaacaga gcaaccagcc aggacccttc
     1561 cagttctccc aggctccaca gcccccgctt ttccagtccc ctcagcccaa gttgccctac
     1621 ctccccactt tccccttctc catgcccagt tcactgacgc ttccaccgcc cgaagactct
     1681 ctctttatgt ttccctgtgg ccccagcggg ggcacatcgc agggctattg cccaggtgcc
     1741 tcctcaggac agatcctgat gcaaccacct gctgggaata taggtacagc ctcctggagt
     1801 gacccctgtt tgccagagct gcccttccct ggtccgttct gcccacaagc tctggggcat
     1861 cccccaggag gggatggcta ctttcctgat ctatttccaa ctccctgccc ccaggctctg
     1921 ggcaggcagc cttcgtcagc tctctcatgg atgcctgaag gggccagacc agggactggg
     1981 cccttactca gcaaggcaaa agaggaacca ccagctgctt ccctggatca gccctcagca
     2041 ctggaggagg ccagagggga tgacaagaat agccatgtcc cctagtaatg aactagtccg
     2101 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaggcacca
     2161 cagttcatcg cgttggc
//
