LOCUS       sequence-280331-        2951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1104
FEATURES             Location/Qualifiers
     source          1..2951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccaggt agcagtgtcc accctgcctg ttgaagaaga gtcctcctca
       61 gagaccagga tggtggtgac attcctcgtg tctgccctcg aatccatgtg taaagaactg
      121 gccaagtcca aggcagaagt ggcctgcatc gcagtgtacg aaacagacgt gtttgtcgtc
      181 ggaaccgaga gaggatgcgc ttttgttaat gccaggacgg attttcagaa agattttgca
      241 aaatactgcg ttgcagaggg actgtgtgag gtgaaacctc cctgccctgt gaacgggatg
      301 caggtccact caggcgaaac ggaaatactc aggaaggcag tggaggacta tttctgcttt
      361 tgttatggta aagccttagg gacaacagtg atggtgcctg ttccctatga gaagatgctg
      421 cgagaccagt cggctgtggt agtgcagggg cttccggaag gcgttgcctt tcaacaccct
      481 gagaattacg accttgcaac cctgaaatgg attttggaga acaaagcagg gatttcattc
      541 atcataaata gacccttcct aggaccagag agtcagctgg gtggccctgg gatggtaaca
      601 gatgcggaga gatccatagt atcaccaagt gaaagctgcg gccccatcaa tgtgaaaact
      661 gaacccatgg aagattctgg catttcactg aaagcagaag ctgtctcagt caagaaagaa
      721 tcagaagatc ctaattacta tcaatataat atgcaaggaa gccacccttc ttccacaagc
      781 aatgaagtaa tagaaatgga attaccaatg gaagattcca ctccgctggt cccttcagaa
      841 gaaccaaatg aggaccctga agccgaggtg aaaatcgaag gaaacacaaa ttcatccagt
      901 gttacaaatt ctgcagcagg tgttgaagat cttaacatcg ttcaagtgac tgttccagat
      961 aatgagaagg aaagattatc aagcattgaa aagattaaac agctaagaga acaagttaat
     1021 gacctcttta gccgaaaatt tggtgaagca attggcgtgg atttccctgt gaaagttccc
     1081 tacaggaaga tcacattcaa ccctggctgt gtggtgattg atggcatgcc cccgggggtg
     1141 gtattcaagg cccccggcta tctggaaatc agttccatga ggaggatctt ggaggcagct
     1201 gagtttatca aattcacagt catcaggccg cttccagggc ttgagctcag taatgtggga
     1261 aaacgcaaga tagaccagga gggccgtgtg tttcaagaaa agtgggagag agcgtatttc
     1321 ttcgtggaag tacagaatat tccaacatgt ctcatatgca aacaaagcat gtctgtgtcc
     1381 aaagaatata acctaagacg ccactatcaa accaatcaca gcaagcatta tgaccagtat
     1441 atggaaagaa tgcgtgacga gaagcttcac gagctgaaaa aagggctcag gaagtatctc
     1501 ttaggctcat cagacaccga gtgtcccgag caaaaacaag tgtttgcaaa cccaagtcca
     1561 acccagaaat cccccgtgca gcctgtagag gacctagctg ggaacttatg ggagaagtta
     1621 cgtgaaaaaa tcaggtcttt tgtggcatat tctatcgcaa tcgatgagat cacggatata
     1681 aataatacca cccagttggc catattcatc cgtggtgtcg atgagaattt cgatgtgtcc
     1741 gaagaacttc tggacacggt gcccatgacg ggtacaaaat ctggcaacga gatcttttcg
     1801 cgtgttgaga aaagcctgaa aaacttctgt atcgactggt cgaaattagt aagcgtggcc
     1861 tccactggca ccccagcgat ggtggatgcc aataacgggc ttgtcacaaa actgaagtcc
     1921 agggtggcga cgttctgcaa gggtgcggaa ctgaagtcca tctgttgtat aattcatccg
     1981 gaatcactct gtgctcagaa gttgaagatg gaccacgtca tggacgtggt agtgaagtcc
     2041 gtgaactgga tatgctcccg gggactgaac cacagtgagt tcacaacctt gctctatgag
     2101 ctggacagcc agtatggtag cctcctgtac tacacggaga ttaagtggct cagtcgcggg
     2161 ctcgtgctaa agagattttt cgaatccttg gaagaaatcg actccttcat gtcatccaga
     2221 gggaaacccc tgcctcaact gagctccata gattggatcc gagacctggc cttcttggtt
     2281 gacatgacga tgcatctgaa cgctttgaac atctctctcc aaggacactc ccaaatcgtc
     2341 acgcagatgt atgacctgat ccgggcgttc ctagcaaaac tgtgcctctg ggagactcat
     2401 ttgacgagga ataatctggc ccactttccc accctgaaat tggcttccag aaatgaaagc
     2461 gatggcctga actacattcc caaaatcgcg gaactccaga ccgaattcca gaaaaggctg
     2521 tctgatttca aactctacga aagcgaactg actctgttca gctccccgtt ctccacgaag
     2581 atcgacagtg tgcacgagga gctccagatg gaggttatcg acctgcaatg caacacggtc
     2641 ctgaagacga aatacgacaa ggtgggaata ccagaattct acaagtacct ctggggtagc
     2701 tacccgaaat acaagcacca ttgcgcaaag attctttcca tgttcgggag cacctacatc
     2761 tgcgaacagc tgttctccat tatgaaactg agcaaaacaa aatactgctc ccagttaaag
     2821 gattcccagt gggattctgt actccacatc gcaacgtagt aatgaactag tccgtaactt
     2881 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgatga gggttacctg
     2941 tgatatccgc g
//
