LOCUS       sequence-280379-        1658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1185
FEATURES             Location/Qualifiers
     source          1..1658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(828..839)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tgtctacagc ctctgccgcc tcctcctcct cctcgtcttc ggccggtgag
       61 atgatcgaag ccccttccca ggtcctcaac tttgaagaga tcgactacaa ggagatcgag
      121 gtggaagagg ttgttggaag aggagccttt ggagttgttt gcaaagctaa gtggagagca
      181 aaagatgttg ctattaaaca aatagaaagt gaatctgaga ggaaagcgtt tattgtagag
      241 cttcggcagt tatcccgtgt gaaccatcct aatattgtaa agctttatgg agcctgcttg
      301 aatccagtgt gtcttgtgat ggaatatgct gaagggggct ctttatataa tgtgctgcat
      361 ggtgctgaac cattgccata ttatactgct gcccacgcaa tgagttggtg tttacagtgt
      421 tcccaaggag tggcttatct tcacagcatg caacccaaag cgctaattca cagggacctg
      481 aaaccaccaa acttactgct ggttgcaggg gggacagttc taaaaatttg tgattttggt
      541 acagcctgtg acattcagac acacatgacc aataacaagg ggagtgctgc ttggatggca
      601 cctgaagttt ttgaaggtag taattacagt gaaaaatgtg acgtcttcag ctggggtatt
      661 attctttggg aagtgataac gcgtcggaaa ccctttgatg agattggtgg cccagctttc
      721 cgaatcatgt gggctgttca taatggtact cgaccaccac tgataaaaaa tttacctaag
      781 cccattgaga gcctgatgac tcgttgttgg tctaaagatc cttcccagcg cccttcaatg
      841 gaggaaattg tgaaaataat gactcacttg atgcggtact ttccaggagc agatgagcca
      901 ttacagtatc cttgtcagta ttcagatgaa ggacagagca actctgccac cagtacaggc
      961 tcattcatgg acattgcttc tacaaatacg agtaacaaaa gtgacactaa tatggagcaa
     1021 gttcctgcca caaatgatac tattaagcgc ttagaatcaa aattgttgaa aaatcaggca
     1081 aagcaacaga gtgaatctgg acgtttaagc ttgggagcct cccgtgggag cagtgtggag
     1141 agcttgcccc caacctctga gggcaagagg atgagtgctg acatgtctga aatagaagct
     1201 aggatcgccg caaccacagc ctattccaag cctaaacggg gccaccgtaa aactgcttca
     1261 tttggcaaca ttctggatgt ccctgagatc gtcatatcag gcaacggaca gccaagacgt
     1321 agatccatcc aagacttgac tgtaactgga acagaacctg gtcaggtgag cagtaggtca
     1381 tccagtccca gtgtcagaat gattactacc tcaggaccaa cctcagaaaa gccaactcga
     1441 agtcatccat ggacccctga tgattccaca gataccaatg gatcagataa ctccatccca
     1501 atggcttatc ttacactgga tcaccaacta caggcaagaa ctagttgcag aactggacca
     1561 ggatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1621 tgtggaaagg acgaccggta tttagagcga cgattgtc
//
