LOCUS       sequence-280520-        1562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1414
FEATURES             Location/Qualifiers
     source          1..1562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagccat ttatttgtcc cgtgggtttt tttccaggga accaatctgc
       61 ccttttgaag aaaagacaaa ggtagaaagg atggtggagg actacctggc aagtggttat
      121 caggattcag tgacgtttga tgatgtggct gtggacttca ccccagagga gtgggcttta
      181 ctggacacaa ctgagaaata cctctacaga gatgtgatgc tggagaacta catgaacctg
      241 gcctctgtgg aatgggaaat acaacctaga accaaacggt catcacttca gcagggtttt
      301 ttgaagaatc aaatattcag tgggatacaa atgacaagag gctacagtgg atggaaactc
      361 tgtgactgta agaattgtgg agaggtcttc agggaacagt tttgccttaa gacacacatg
      421 agagttcaga atggagggaa tacttctgag ggtaattgtt atggaaaaga caccctcagt
      481 gtgcacaagg aagcctctac tggacaggaa ctttccaaat ttaatccatg tggaaaagtc
      541 tttactctaa ctccaggtct tgctgtacat cttgaagttc tcaatgcaag acaaccctac
      601 aaatgtaagg aatgtggaaa aggctttaag tattttgcaa gccttgataa tcatatggga
      661 atccacactg atgagaaact ctgtgaattt caggaatatg ggagagctgt cacagcttct
      721 tcacacctaa agcagtgtgt agcagttcat acaggaaaga aatccaaaaa gactaagaaa
      781 tgtgggaaat ccttcactaa tttttctcaa ctttatgcac ctgtgaaaac tcataaagga
      841 gagaagtcct ttgaatgtaa agaatgtgga agatccttta gaaattcctc atgccttaat
      901 gatcacattc aaattcacac tggaataaaa ccacacaagt gtacttactg tgggaaagcc
      961 ttcactagat caactcaact tactgaacat gtaagaactc acactggaat aaaaccctat
     1021 gaatgtaagg aatgtggcca agcctttgct cagtactcgg gcctttctat acacatacga
     1081 agtcacagtg gaaagaaacc ctatcagtgt aaggaatgtg ggaaagcctt cactacatcc
     1141 acaagtctta ttcagcatac aagaattcac acaggagaga agccttatga atgtgttgaa
     1201 tgtgggaaga ccttcattac ttcttcccgt cgtagtaaac atttgaaaac tcatagtgga
     1261 gaaaagccct ttgtatgcaa gatatgtggg aaagcatttc tatattcctc acgccttaat
     1321 gttcacctgc gaactcatac cggagagaaa cccttcgtat gtaaagaatg tgggaaagca
     1381 tttgctgttt cctcacgcct aagtagacat gaaagaattc acactgggga gaaaccctat
     1441 gaatgcaagg atatgagtgt taccatttag taatgaacta gtccgtaact tgaaagtatt
     1501 tcgatttctt ggctttatat atcttgtgga aaggacgagg cccattgcga ggcccagctg
     1561 ta
//
