LOCUS       sequence-280541-        2351 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1474
FEATURES             Location/Qualifiers
     source          1..2351
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggtggt gccagctgag gtgaatagtt tgcttccaga ggaaataatg
       61 gacactggta taactttagt ggatgatgat agtattgagg ctgttattgt ttcatcccca
      121 attcccatgg agacagaact ggaagaaatt gtcaacataa attctactgg tgactctaca
      181 gccacgccca tttccacgga accaatcaca gtgtacagta accacactaa ccaagttgca
      241 gtgaatacca caattactaa agcagattct aataccacag tgaaaccagc ttttccaagt
      301 ggccttcaaa aacttggtgc tcagactcct gtgactatat cagccaatca gattatttta
      361 aacaaagtat cacagacatc tgatcttaaa cttggcaatc agacccttaa accagatgga
      421 cagaagttaa ttttaacaac tttgggcaag tctggttcac caattgtttt agcactaccc
      481 catagccaac taccccaggc tcagaaagtt acaactcagg cccagtcagg agatgctaag
      541 ttaccaccgc agcaaattaa agtagttacc attggaggga ggccagaggt gaaacctgtc
      601 attggtgtct cagcattgac cccaggaagt caactgatta atactacaac tcagccctct
      661 gtgttacaga cccaacagtt aaaaacagta cagattgcta agaagcctcg aacgccaacc
      721 tctggtccag taatcacgaa gctgatcttt gcaaaaccaa ttaatagtaa agcagttaca
      781 ggacagacaa ctcaagtttc accaccagtt attgcaggta gggttctttc acagtctact
      841 cccggaactc catcaaagac cataacaata tctgaaagtg gtgttattgg atcaacttta
      901 aattctacaa cacagacacc aaataaaata gccatctcac ctttgaaatc gccaaataag
      961 gcagtgaaat caactgtgca gaccatcact gttggaggag tgagcacatc acagtttaag
     1021 acaattattc ctctggcaac tgctcccaat gtccagcaga ttcaagtgcc tggaagcaag
     1081 tttcattatg tccgacttgt tactgccaca tcagccagta gctcaaccca gccagttagt
     1141 cagaatccca gtacaaacac tcagcctctt cagcaagcaa agccagtggt tgttaataca
     1201 accccagtgc ggatgtcagt tccaattgtc tcagctcagg ctgtcaaaca agttgttcca
     1261 aaaccaatca atccaacttc acaaatagta actactagcc agccacagca acggcttatc
     1321 atgcctgcca caccactgcc acagatccag cccaacctca ctaacctgcc accaggcact
     1381 gtcctggcac cagctccggg aacagggaat gtgggttatg cagtgcttcc agctcagtat
     1441 gttactcagc tacagcagtc ttcatatgta tcaatagcaa gcaactctac ctttactgga
     1501 acatctggta tccagaccca ggcacggctt ccattcaatg gcataatccc atcagagtcg
     1561 gccagtcggc cccgaaagcc ctgtaattgt acaaaatcac tgtgtttgaa attgtattgt
     1621 gattgctttg caaatggtga attttgcaac aactgcaatt gtactaattg ttacaacaat
     1681 ttggaacatg aaaatgaaag gcaaaaagca ataaaggcat gccttgacag aaatccagaa
     1741 gcctttaagc ctaagatagg gaaaggaaag gagggagaat ctgatcgacg tcatagcaaa
     1801 gggtgtaatt gcaaacgatc aggatgtctt aaaaactact gtgaatgcta tgaggcaaaa
     1861 ataatgtgtt cctcaatatg caaatgtatt ggctgtaaga attttgaaga aagcccggaa
     1921 aggaagacat tgatgcattt ggcagatgca gctgaagtaa gggtacagca acaaacagca
     1981 gccaagacga agttatcctc tcaaatttca gacttgctta ctaggccaac accagcttta
     2041 aatagtggag gcggaaaatt gccatttaca tttgtaacta aggaagtagc tgaagccaca
     2101 tgtaattgcc tccttgccca ggcagagcag gcagacaaga agggaaaatc aaaggcagca
     2161 gcggaacgga tgatacttga ggaattcgga cgatgtttga tgagtgtcat caactctgca
     2221 ggaaaggcaa aaagtgaccc ttgtgccatg aattgctagt aatgaactag tccgtaactt
     2281 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagat atggatcccg
     2341 ttaatgcgcg g
//
