LOCUS       sequence-280571-        1724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1616
FEATURES             Location/Qualifiers
     source          1..1724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaactctt aacattcagg gatgtggcca tagaattctc tccagaagag
       61 tggaaatgcc tagaccctgc ccaacagaat ttatatagag atgtgatgtt ggagaactac
      121 agaaacctcg tctccctggc tgtgtattct tattacaacc aaggcatttt accagagcaa
      181 ggcatacaag attcattcaa aaaagcaaca ctgggaagat atgggagctg tggccttgaa
      241 aatatatgct tatggaaaaa ctgggaaagt ataggtgaag gtgaaggaca aaaggaatgt
      301 tataatctat gtagccaata tttgacaact agtcataaca aacatttaac tgtgaaagga
      361 gacaaagaat atagaatatt tcagaagaag cctcagtttc tgtcagctgc tcctacagaa
      421 ccatgtattc ctatgaataa atatcaacat aaatttttga aatctgtctt ttgtaataaa
      481 aatcagataa attttaacca tgactcaaat attagtaaac atcatagtac tcattttcta
      541 gaaaactatt acaattgtaa tgaatgtgaa aaagtatttt accaatcctc aaagcttatt
      601 ttccctgaaa atatccatat tcaaaaaaag ccttacaact ctaatgaatg tggtgaaact
      661 tctgacccat tctcaaagct tactcaacat caaagaattt atattggaga gagctcacaa
      721 agatgtaata aaaaatgtat aatagtcttt agtcaatcac atctaaaggg acataagata
      781 attaacactg gagaaaaatc agtgaaatat aaagaacgtg gcaaagcttt taccaggggc
      841 ttacatcttg gacatcagaa aattcatact ggagagaaac cttacaaatg taaaaaatgt
      901 gacaaagcct ttaacaagag ttcacacctt gctcaacatc agagaatcca tactggagag
      961 aagcctttca agtgtaagga atgtggcaaa gcttttaaca gaggctcata ccttactcaa
     1021 catcagagaa tccatactgg agagagagct ttcaaatgtg aagaatgtgg caaagccttt
     1081 aacagggggt cataccttac tcaacaccag agaatccata ccggagagaa acctttcaga
     1141 tgtaaggaat gtggcaaagc ctttaataga agctcatacg ttactcagca tcagagaatg
     1201 catactggag agaaaccttt caagtgtaag gaatgtggca aagcttttaa cagagcttca
     1261 caccttactc aacatcagag aatccatact ggagagaaac acttcaaatg taaagaatgt
     1321 ggcaaagcct ttaacagggg ctcacacctt actcgacatc aaagaatcca tactggagag
     1381 aagtctttca aatgtgaaga atgtggcaaa gcttttatct ggggctcaca ccttactcaa
     1441 catcagagag tccatactgg agagaaattc ttcaaatgta aagaatgtgg caaagctttt
     1501 accaggagct cacaccttac tcaacatcag agaattcata ctggagagaa acctttcaaa
     1561 tgtaaagaat gtggcaaagc ttttaacaga cgctcaaccc ttactcaaca tcaaataatt
     1621 cataccaggt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1681 atatcttgtg gaaaggacga gatgtcgtgc tgtgaaatat cgcg
//
