LOCUS       sequence-280652-        1649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1729
FEATURES             Location/Qualifiers
     source          1..1649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggactatt ggcattcagg gatgtggctc tagaattctc tccagaggag
       61 tgggaatgcc tggacccagc tcagcggagt ttgtataggg atgtgatgtt agagaactac
      121 agaaacctga tctcccttgg tgaggatagc ttcaatatgc aattcctatt tcacagtctt
      181 gctatgtcta agccagaact gatcatctgt ctggaggcaa ggaaagagcc ctggaacgtg
      241 aacacagaga agacagccag acactcagtt ttgtcttctt atcttactga agacattttg
      301 ccagagcagg gcctgcaagt ttcattccaa aaagtgatgc tgagaagata tgaaagatgt
      361 tgtcttgaga aattacgctt aaggaatgac tgggaaattg tgggtgagtg gaaagggcag
      421 aaggcaagtt ataatggact tgacctatgc tcagcaacta ctcatagcaa aaactttcaa
      481 tgcaataaat gtgtgaaagg ttttagtaaa tttgcaaatc taaataaatg taagataagc
      541 catactggag aaaaaccatt caaatgcaaa gaatgtggca atgtctcttg catgtcttta
      601 ataatgactc aacagcagag aatccatatt ggagagaacc cgtaccaatg taaaaaatgt
      661 ggcaaagcct ttaatgagtg ctcatgcttt actgactgta agagaattca tgttggagag
      721 aaacattgca aatgtgaaga atgtaataac atttttaagt cttgctcaag tcttgctgtt
      781 gttgagaaaa atcatactga aaagaaaacc tacagatgtg aagaatgtgg caaagctttt
      841 aacctgtgct cagttcttac taaacataag aaaattcata ctggagagaa accatacaaa
      901 tgtgaagaat gtggcaaatc ctttaagttg ttcccatacc ttactcaaca caagagaatt
      961 catagtagag agaaacccta caagtgtgaa gaatgtggca aagtctttaa attgttgtca
     1021 taccttactc aacatagaag aattcatact ggagagaaaa ccttccgatg tgaagaatgt
     1081 ggaaaagcct ttaaccagag ctcacatctg actgaacata ggagaattca tactggtgag
     1141 aaaccataca aatgtgagga atgtggcaaa gcttttacct ggttctcata ccttattcag
     1201 cataagagaa ttcatactgg gcagaaaccc tacaaatgtg aggaatgtgg caaagctttt
     1261 acctggtttt cataccttac tcaacataag agaattcata ctggagagaa accctacaaa
     1321 tgtgatgaat gtggcaaagc ttttaactgg ttttcatatc ttactaatca taagagaatt
     1381 catactggag agaaacccta caaatgtgaa gaatgtggca aagcctttgg ccagagctca
     1441 cacctttcta aacataagac aattcatacc agagagaaac catacaagtg tgaggaatgt
     1501 ggcaaagcct ttaaccactc tgcacaactt gctgtacatg agaaaactca tacctagtaa
     1561 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1621 gacgataata cacctcatgg gtcatggtc
//
