LOCUS       sequence-280697-        1436 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1780
FEATURES             Location/Qualifiers
     source          1..1436
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaccatt gcaatttaga gatgtggcca tagaattctc tctggaggag
       61 tggcattgcc tggacgctgc acagcggaat ctatataggg atgtgatgtt agagaactac
      121 agaaacctga tcttccttgg tattgttgtc tctaaaccaa acctgatcac ctgtctggag
      181 caaggaaaaa aacctttaac tatgaagagg catgagatga ttgccaaacc cccagttatg
      241 tattctcatt ttgcccaaga cctttggtca gagcagagca taaaagattc tttccaaaaa
      301 gtgatactaa gaagatatga aaaatgtaga catgacaatt tacagttaaa aaaaggctgt
      361 gaaagtgtag atgagtgtcc agtgcacaaa agaggttata atggacttaa acaatgtttg
      421 gcaactaccc agagaaaaat atttcaatgt gatgaatatg tgaaattctt gcataaattt
      481 tcaaattcaa acaaacataa gataagagat actggaaaaa aatcttttaa atgtatagaa
      541 tatgggaaaa cttttaacca gtcttcaacc cgtactacat ataagaaaat tgatgctgga
      601 gagaaacgct ataaatgtga agaatgtggt aaagcctata agcagtcctc acaccttact
      661 acacataaga aaattcatac tggagagaaa ccctacaaat gtgaagaatg tggcaaagcc
      721 tataagcagt cctgtaacct tactacacat aagataattc atactggaga gaaaccctac
      781 agatgtagag aatgtggcaa agcttttaac caccctgcaa cccttttttc acataagaaa
      841 attcatactg gagagaaacc atacaagtgt gataaatgtg gcaaagcctt tatttcatcc
      901 tcaaccctta ctaaacatga gataattcat actggagaga aaccctacaa atgtgaggaa
      961 tgtggcaaag cttttaaccg ttcctcaaac cttactaaac ataagagaat tcatactgga
     1021 gatgtaccct acaaatgtga cgaatgtggc aaaaccttta cctggtactc aagcctctct
     1081 aaacataaga gagctcatac tggagagaaa ccctacaagt gtgaagaatg tggcaaagcc
     1141 tttactgcat tctcaactct aactgaacat aagataattc atactggaga gaaaccgtac
     1201 aaatgtgaag aatgtggcaa agcttttaac tggtcctcag cccttaataa acataagaaa
     1261 attcatatta gacagaaacc ctgcatagtg aagaatgtgg aaaatctttt aaatgttcct
     1321 caacccttaa taagcataag atagtaatga actagtccgt aacttgaaag tatttcgatt
     1381 tcttggcttt atatatcttg tggaaaggac gactctgagt ccttcgaccg aaatta
//
