LOCUS       sequence-280712-        1259 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1801
FEATURES             Location/Qualifiers
     source          1..1259
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagcctt cctatctgct ggcctcggca tacttgcacc ctcagagacc
       61 taccccctac ctacaaccag ctctggctgg gagccccggc tggggtcacc attcccatca
      121 ggcccttgca ccagctctac tggggcccaa gctgtggccg agcccactgg gcagggcccc
      181 aagaacccac gtgtgtccag agtgacagtt cagctggaga tgaagcctct gtgggaggaa
      241 ttcaaccagc tgggcactga gatgatcgtc accaaggcag gcaggaggat gttccccccc
      301 ttccaggtga agatcctggg catggactcc ctggccgact acgccctgct catggacttc
      361 atccccctgg acgacaagag atacaggtat gccttccaca gctcggcctg gctggtggcg
      421 ggcaaggcag acccagccac acctggccgc gtgcacttcc accccgactc gccagccaag
      481 ggtgcccagt ggatgcgcca gattgtgtcc tttgacaagc tcaagctgac caacaacctg
      541 ctggatgaca atggccacat cattctcaac tctatgcacc gctaccagcc ccgtttccac
      601 gtggtcttcg tggacccacg caaggacagt gagcgctatg cccaggagaa cttcaagtcc
      661 ttcatcttca cagagaccca gttcacagca gtgacagcct atcagaacca caggatcacc
      721 cagctgaaaa tcgccagcaa cccttttgcc aaaggcttta gagagagtga cctggactcc
      781 tggcctgtgg ccccacggcc cctgctcagt gtcccagccc ggagtcacag cagcctcagt
      841 ccctgtgtgc tgaagggtgc cacagacagg gagaaagacc ccaacaaagc ttcagcttcc
      901 acctccaaga cccctgcttg gctccatcat cagctgctgc ccccacctga ggtcctgctg
      961 gccccggcca cctacaggcc tgtcacgtat cagagcctgt actctggagc cccgagccac
     1021 ctagggatcc caaggacccg accagcacca taccccctcc ccaacatccg ggctgatagg
     1081 gatcaaggag gcctgcctct cccagctggg ctggggctcc tgtcccccac tgtggtgtgc
     1141 ctggggcctg gccaggactc ccagtagtaa tgaactagtc cgtaacttga aagtatttcg
     1201 atttcttggc tttatatatc ttgtggaaag gacgaaataa gtgcggatcg ccgcggagt
//
