LOCUS       sequence-280730-        2225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1836
FEATURES             Location/Qualifiers
     source          1..2225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggccagac tgggaagaaa tctgagaagg gaccagtttg ttggcggaag
       61 cgtgtaaaat cagagtacat gcgactgaga cagctcaaga ggttcagacg agctgatgaa
      121 gtaaagagta tgtttagttc caatcgtcag aaaattttgg aaagaacgga aatcttaaac
      181 caagaatgga aacagcgaag gatacagcct gtgcacatcc tgacttctgt gagctcattg
      241 cgcgggacta gggaggtgga agatgaaact gttttacata acattcctta tatgggagat
      301 gaagttttag atcaggatgg tactttcatt gaagaactaa taaaaaatta tgatgggaaa
      361 gtacacgggg atagagaatg tgggtttata aatgatgaaa tttttgtgga gttggtgaat
      421 gcccttggtc aatataatga tgatgacgat gatgatgatg gagacgatcc tgaagaaaga
      481 gaagaaaagc agaaagatct ggaggatcac cgagatgata aagaaagccg cccacctcgg
      541 aaatttcctt ctgataaaat ttttgaagcc atttcctcaa tgtttccaga taagggcaca
      601 gcagaagaac taaaggaaaa atataaagaa ctcaccgaac agcagctccc aggcgcactt
      661 cctcctgaat gtacccccaa catagatgga ccaaatgcta aatctgttca gagagagcaa
      721 agcttacact cctttcatac gcttttctgt aggcgatgtt ttaaatatga ctgcttccta
      781 catccttttc atgcaacacc caacacttat aagcggaaga acacagaaac agctctagac
      841 aacaaacctt gtggaccaca gtgttaccag catttggagg gagcaaagga gtttgctgct
      901 gctctcaccg ctgagcggat aaagacccca ccaaaacgtc caggaggccg cagaagagga
      961 cggcttccca ataacagtag caggcccagc acccccacca ttaatgtgct ggaatcaaag
     1021 gatacagaca gtgataggga agcagggact gaaacggggg gagagaacaa tgataaagaa
     1081 gaagaagaga agaaagatga aacttcgagc tcctctgaag caaattctcg gtgtcaaaca
     1141 ccaataaaga tgaagccaaa tattgaacct cctgagaatg tggagtggag tggtgctgaa
     1201 gcctcaatgt ttagagtcct cattggcact tactatgaca atttctgtgc cattgctagg
     1261 ttaattggga ccaaaacatg tagacaggtg tatgagttta gagtcaaaga atctagcatc
     1321 atagctccag ctcccgctga ggatgtggat actcctccaa ggaaaaagaa gaggaaacac
     1381 cggttgtggg ctgcacactg cagaaagata cagctgaaaa aggacggctc ctctaaccat
     1441 gtttacaact atcaaccctg tgatcatcca cggcagcctt gtgacagttc gtgcccttgt
     1501 gtgatagcac aaaatttttg tgaaaagttt tgtcaatgta gttcagagtg tcaaaaccgc
     1561 tttccgggat gccgctgcaa agcacagtgc aacaccaagc agtgcccgtg ctacctggct
     1621 gtccgagagt gtgaccctga cctctgtctt acttgtggag ccgctgacca ttgggacagt
     1681 aaaaatgtgt cctgcaagaa ctgcagtatt cagcggggct ccaaaaagca tctattgctg
     1741 gcaccatctg acgtggcagg ctgggggatt tttatcaaag atcctgtgca gaaaaatgaa
     1801 ttcatctcag aatactgtgg agagattatt tctcaagatg aagctgacag aagagggaaa
     1861 gtgtatgata aatacatgtg cagctttctg ttcaacttga acaatgattt tgtggtggat
     1921 gcaacccgca agggtaacaa aattcgtttt gcaaatcatt cggtaaatcc aaactgctat
     1981 gcaaaagtta tgatggttaa cggtgatcac aggataggta tttttgccaa gagagccatc
     2041 cagactggcg aagagctgtt ttttgattac agatacagcc aggctgatgc cctgaagtat
     2101 gtcggcatcg aaagagaaat ggaaatccct tagtaatgaa ctagtccgta acttgaaagt
     2161 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaggccaaag ctattgtgga
     2221 cgctg
//
