LOCUS       sequence-280973-        3308 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2452
FEATURES             Location/Qualifiers
     source          1..3308
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtctctag cgaccggccc gtgtcactgg aggacgaggt ctcccatagt
       61 atgaaggaga tgattggagg ctgttgcgtt tgctcagacg agagaggctg ggccgagaac
      121 ccgctggttt attgcgacgg gcacggctgc agcgtcgcgg tgcatcaagc ttgctatggc
      181 attgttcaag tacccactgg accgtggttt tgcaggaaat gtgaatctca ggagagagca
      241 gccagagtga gatgtgaact ttgtccccat aaggatggag ctttaaaaag aacagataat
      301 gggggttggg cccatgtggt ttgtgccctg tatattccag aggtacaatt tgccaatgtt
      361 tccacaatgg aaccaattgt tttacagtct gttccgcatg atcgttataa taagacttgc
      421 tacatttgtg atgaacaagg aagagaaagc aaagcagcca ctggtgcttg catgacatgt
      481 aataaacatg gatgtcgaca ggctttccat gtaacatgcg ctcagtttgc cggactgctt
      541 tgtgaagaag aaggtaatgg tgccgataat gtccaatact gtggctactg taaataccat
      601 tttagtaagc tgaaaaagag caaacgggga tctaataggt catatgatca aagtttaagt
      661 gattcttcct ctcactctca ggataaacat catgagaaag agaaaaaaaa atataaagag
      721 aaggacaaac acaaacagaa acacaagaag cagccagaac catcacctgc attggttcca
      781 tccttgactg ttactacaga aaaaacttat acaagcacta gcaacaactc tatatctgga
      841 tcattgaagc gcttggaaga tactactgca cgatttacaa atgcaaattt ccaggaagtc
      901 tctgcacaca cctctagtgg aaaagatgtt tcagagacta gagggtcaga gggcaaaggg
      961 aagaaatctt cagctcacag ctcaggtcaa aggggaagaa agcctggtgg tggaagaaat
     1021 ccaggaacaa ctgtgtcagc agctagccct tttcctcaag gcagtttttc aggaactcca
     1081 ggcagtgtaa agtcatcttc tggaagttca gtgcagtctc cccaggattt cctgagcttt
     1141 acagactcag atctgcgtaa tgacagttac tctcactccc aacagtcatc agcaaccaaa
     1201 gatgtacata aaggagagtc tggaagccag gaaggggggg taaatagttt tagtacctta
     1261 attggcctcc cttcaacctc agctgttact tcacagccta aaagctttga aaattcacct
     1321 ggagatttgg gtaattccag ccttcctaca gcaggatata agcgggctca aacttctggc
     1381 atagaagaag aaactgtaaa ggaaaagaaa aggaaaggaa ataaacaaag taagcatggg
     1441 cctggcagac ccaaaggaaa caaaaatcaa gagaatgttt ctcatctctc agtttcttct
     1501 gcttcaccaa catcatctgt agcatcagct gcaggaagca taacaagctc tagtctgcag
     1561 aaatctccta cattgctcag gaatggaagt ttacagagcc tcagtgttgg ctcatctcca
     1621 gttggttcag aaatttccat gcagtatcgg catgatggag cttgcccaac aactacgttc
     1681 tcagagttgc tgaatgcaat acacaacggt atttataaca gcaatgatgt agcagtatcg
     1741 tttccaaatg tagtatctgg ctcgggatct agtactcctg tctccagctc tcacttacct
     1801 cagcagtctt ctgggcattt gcaacaagta ggagcgctct ctccctcagc tgtgtcatct
     1861 gcagcccctg ctgttgctac aactcaggca aatactctat ctggatcttc tctcagtcag
     1921 gcaccatctc atatgtatgg caatagatca aattcatcaa tggcagctct tatagctcag
     1981 tctgaaaaca atcaaacaga tcaagatctt ggagacaata gccgcaacct agttggcaga
     2041 ggaagctcac cccgaggaag tctctcgcca cgatcccctg taagcagctt acagattcgc
     2101 tatgatcaac caggcaacag cagtttggaa aatctgcctc cagtagcagc cagcatagaa
     2161 cagcttttgg agaggcagtg gagtgaagga cagcaatttt tactagaaca gggtactcct
     2221 agtgacattt taggaatgct gaagtcatta caccaacttc aagttgaaaa ccgaagatta
     2281 gaggaacaaa ttaaaaactt gactgccaaa aaggaacggc ttcagttatt gaatgcacag
     2341 ctttcagtgc cttttccaac aataacagca aatcctagtc cgtctcatca aatacacaca
     2401 ttttcagcac agactgctcc tactactgat tccttgaaca gcagtaagag ccctcatata
     2461 ggaaacagct ttttacctga taattctctt cctgtattaa atcaggactt aacctccagt
     2521 ggacaaagta ccagcagctc atcagctctt tctaccccac ctcctgctgg gcagagtccg
     2581 gctcaacaag gctcaggagt gagtggagtt cagcaggtca atggcgtgac agtgggggca
     2641 ctagctagtg gaatgcagcc tgtaacttcc accattcctg ccgtgtctgc agtgggtgga
     2701 ataattggag ctttgccagg taaccaactg gcaattaatg gcattgtagg agctttaaat
     2761 ggggttatgc agactcctgt cacaatgtcc cagaacccta cccctctcac ccacacaacc
     2821 gtaccaccta atgcaacaca tccaatgcca gctacactga ctaacagtgc ctcaggacta
     2881 ggattacttt ctgaccagca acgacaaata cttattcatc aacagcagtt tcagcagttg
     2941 ttaaattctc aacagctcac accagaacaa catcaagcct ttttgtatca gttaatgcaa
     3001 catcaccacc agcagcacca ccaacctgaa cttcagcagc tgcagatccc tggaccaaca
     3061 caaataccca taaacaacct tcttgcaggt acacaggcac ccccacttca cacagctacc
     3121 accaacccat ttctcaccat ccatggagat aatgcaagtc agaaagtagc aagacttagt
     3181 gataaaactg ggcctgtagc tcaagagaaa agttagtaat gaactagtcc gtaacttgaa
     3241 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaagcctc ctcatcttac
     3301 ggtactcc
//
