LOCUS       sequence-28099-e         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Levskaya A, Weiner OD, Lim WA, Voigt CA
  TITLE     Spatiotemporal control of cell signalling using a light-switchable 
            protein interaction.
  JOURNAL   Nature. 2009 Oct 15;461(7266):997-1001.
  PUBMED    19749742
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2009-10-15"; volume: "461(7266)"; pages: "997-1001"
COMMENT     Sequence Label: pAL197
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      121..130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    138..162
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      443..578
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt
       61 gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca
      121 ccatggtggc tgagctcacc actttgtaca agaaagctgg gtcggcgcgc ccaccctttg
      181 acttatctgt aaagctcatg tatttctggc tgttggatgt cttcttcgag ttgtaaaact
      241 ccaacacatc cagaacagcc tgcgggtttt tcttctgctc cgacttagtg atatttgatg
      301 tctgaagcaa gcgggcccac tgctctggca ttcccgtaaa ctcccctgtg acagcatcaa
      361 aaccgacatg aattgtgtgt tcaaaatctg aagggagaga aatctctggc cgctctttct
      421 ctttcttcat ggtaccagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
      481 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
      541 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggac
//
