LOCUS       sequence-28102-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PhyB-mCFP with prenylation sequence from Kras, for 
            red-light-induced recruitment of PIF to the plasma membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Levskaya A, Weiner OD, Lim WA, Voigt CA
  TITLE     Spatiotemporal control of cell signalling using a light-switchable 
            protein interaction.
  JOURNAL   Nature. 2009 Oct 15;461(7266):997-1001.
  PUBMED    19749742
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2009-10-15"; volume: "461(7266)"; pages: "997-1001"
COMMENT     Sequence Label: pAL204
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(93..111)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(94..111)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(131..148)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    137..361
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      407..835
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(494..513)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     704..725
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(844..864)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 caaggctagc ggtaaaaaga agaaaaagaa gtcaaagaca aagtgtgtaa ttatgtaagc
       61 ggccgctcga gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct
      121 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      181 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
      241 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
      301 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
      361 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
      421 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      481 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      541 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      601 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
      661 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      721 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
      781 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
      841 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagcn gcagaagtat
      901 gcaaagcatg catctcaatt agtcagcaac cnntgtggaa agtccccagg ctccccagca
      961 ggcagaagta tgcaaagcat gcatctcaat tagtcaggca
//
