LOCUS       sequence-281153-        1829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0042
FEATURES             Location/Qualifiers
     source          1..1829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccatgtc ccaggaatca ttgaccttca aggacgtgtt tgtggacttc
       61 accctggagg agtggcagca actggactct gcccagaaga acctctacag ggatgtcatg
      121 cttgagaact acagccacct ggtgtccgtg gggcatctag ttgggaagcc agatgtgatc
      181 ttcaggttgg gaccaggtga cgagtcctgg atggcagatg gagggacccc ggtacggacc
      241 tgtgcagtct gggaagttga cgagcagata gatcactaca aggaaagcca agacaaattt
      301 ctgtggcaag ctgcattcat aggcaaggaa acactgaagg atgaaagtgg tcaagaatgt
      361 aaaatatgta gaaaaatcat ttatctcaac acagactttg tttctgtaaa acaaagactc
      421 cctaaatatt attcatggga aaggtgttca aaacatcatt taaactttct tggtcaaaat
      481 agaagctatg taagaaagaa agatgatgga tgtaaggcat attggaaagt atgcctccat
      541 tataatcttc ataaagctca acctgcagag agattttttg accctaatca acgagggaaa
      601 gccctccacc aaaagcaagc ccttagaaaa agtcagagaa gtcaaactgg ggagaaactc
      661 tacaaatgta ctgaatgtgg aaaagtgttt atccagaaag caaacttagt tgtacatcaa
      721 agaactcaca ccggagagaa accttatgaa tgctgcgaat gtgcaaaagc cttcagccag
      781 aagtcaaccc tcatagcaca ccagagaact cacacagggg agaagcccta tgaatgcagt
      841 gaatgtggaa aaacctttat ccagaagtca actctgatta aacatcaacg aactcataca
      901 ggagagaaac catttgtatg tgacaaatgt ccaaaagcct ttaagagttc atatcatctt
      961 attagacatg aaaaaacaca cattagacaa gcattttata aaggtattaa atgtactacg
     1021 tccagcctta tatatcaaag aattcacaca agtgagaaac ctcagtgcag tgaacatggg
     1081 aaagcctctg atgagaagcc cagtcccact aaacattgga gaactcatac aaaagagaac
     1141 atttatgagt gtagtaaatg tgggaaaagc ttcagaggaa agtcacacct ctctgttcat
     1201 cagagaattc atacaggaga gaaaccctat gaatgtagta tatgtgggaa gactttcagt
     1261 ggaaagtcac acctctctgt ccatcataga actcatacag gagagaaacc ttatgaatgc
     1321 agaagatgtg ggaaagcctt tggggagaag tcaaccctta ttgtacatca gagaatgcat
     1381 acaggagaga aaccctataa atgcaacgaa tgtgggaaag cctttagtga gaagtcacca
     1441 ctgattaaac atcagagaat tcatacagga gagagaccct atgaatgcac tgactgtaaa
     1501 aaagccttca gtaggaagtc aactctcatc aaacatcaga gaattcatac aggagaaaaa
     1561 ccttacaaat gtagtgaatg tgggaaggcc ttcagtgtga aatcaactct cattgtgcat
     1621 cacagaactc atacaggaga gaaaccttat gaatgcagag actgtgggaa agcattcagt
     1681 gggaagtcaa ctctcattaa acatcagaga agtcacacag gagataaaaa cctatagtaa
     1741 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1801 gacgagaggt acagtgtagg tctctcacc
//
