LOCUS       sequence-281165-        3476 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0093
FEATURES             Location/Qualifiers
     source          1..3476
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggatgg ccccaggtgt aagcgcagaa agcaggcgaa cccgcggcgc
       61 aataacgtta caaattataa tactgtggta gaaacaaatt cagattcaga tgatgaagac
      121 aaactgcata ttgtggaaga agaaagtgtt acagatgcag ctgactgtga aggtgtacca
      181 gaggatgacc tgccaacaga ccagacagtg ttaccaggga ggagcagtga aagagaaggg
      241 aatgctaaga actgctggga ggatgacaga aaggaagggc aagaaatcct ggggcctgaa
      301 gctcaggcag atgaagcagg atgtacagta aaagatgatg aatgcgagtc agatgcagaa
      361 aatgagcaaa accatgatcc taatgttgaa gagtttctac aacaacaaga cactgctgtc
      421 atttttcctg aggcacctga agaggaccag aggcagggca caccagaagc cagtggtcat
      481 gatgaaaatg gaacaccaga tgcattttca caattactca cctgtccata ttgtgataga
      541 ggctataaac gctttacctc tctgaaagaa cacattaaat atcgtcatga aaagaatgaa
      601 gataacttta gttgctccct gtgcagttac acctttgcat acagaaccca acttgaacgt
      661 cacatgacat cacataaatc aggaagagat caaagacatg tgacgcagtc tgggtgtaat
      721 cgtaaattca aatgcactga gtgtggaaaa gctttcaaat acaaacatca cctaaaagag
      781 cacttaagaa ttcacagtgg agagaagcca tatgaatgcc caaactgcaa gaaacgcttt
      841 tcccattctg gctcctatag ctcacacata agcagtaaga aatgtatcag cttgatacct
      901 gtgaatgggc gaccaagaac aggactcaag acatctcagt gttcttcacc gtctctttca
      961 gcatcaccag gcagtcccac acgaccacag atacggcaaa agatagagaa taaacccctt
     1021 caagaacaac tttctgttaa ccaaattaaa actgaacctg tggattatga attcaaaccc
     1081 atagtggttg cttcaggaat caactgttca acccctttac aaaatggggt tttcactggt
     1141 ggtggcccat tacaggcaac cagttctcct cagggcatgg tgcaagctgt tgttctgcca
     1201 acagttggtt tggtgtctcc cataagtatc aatttaagtg atattcagaa tgtacttaaa
     1261 gtggcggtag atggtaatgt aataaggcaa gtgttggaga ataatcaagc caatcttgca
     1321 tccaaagaac aagaaacaat caatgcttca cccatacaac aaggtggcca ttctgttatt
     1381 tcagccatca gtcttccttt ggttgatcaa gatggaacaa ccaaaattat catcaactac
     1441 agtcttgagc agcctagcca acttcaagtt gttcctcaaa atttaaaaaa agaaaatcca
     1501 gtcgctacaa acagttgtaa aagtgaaaag ttaccagaag atcttactgt taagtctgag
     1561 aaggacaaaa gctttgaagg gggggtgaat gatagcactt gtcttctgtg tgatgattgt
     1621 ccaggagata ttaatgcact tccagaatta aagcactatg acctaaagca gcctactcag
     1681 cctcctccac tccctgcagc agaagctgag aagcctgagt cctctgtttc atcagctact
     1741 ggagatggca atttgtctcc tagtcagcca cctttaaaga acctcttgtc tctcctaaaa
     1801 gcatattatg ctttgaatgc acaaccaagt gcagaagagc tctcaaaaat tgctgattca
     1861 gtaaacctac cactggatgt agtaaaaaag tggtttgaaa agatgcaagc tggacagatt
     1921 tcagtgcagt cttctgaacc atcttctcct gaaccaggca aagtaaatat ccctgccaag
     1981 aacaatgatc agcctcaatc tgcaaatgca aatgaacccc aggacagcac agtaaatcta
     2041 caaagtcctt tgaagatgac taactcccca gttttaccag tgggatcaac caccaatggt
     2101 tccagaagta gtacaccatc cccatcacct ctaaaccttt cctcatccag aaatacacag
     2161 ggttacttgt acacagctga gggtgcacaa gaagagccac aagtagaacc tcttgatctt
     2221 tcactaccaa agcaacaggg agaattatta gaaaggtcaa ctatcactag tgtttaccag
     2281 aacagtgttt attctgtcca ggaagaaccc ttgaacttgt cttgcgcaaa aaaggagcca
     2341 caaaaggaca gttgtgttac agactcagaa ccagttgtaa atgtaatccc accaagtgcc
     2401 aaccccataa atatcgctat acctacagtc actgcccagt tacccacaat cgtggccatt
     2461 gctgaccaga acagtgttcc atgcttaaga gcgctagctg ccaataagca aacgattctg
     2521 attccccagg tggcatacac ctactcaact acggtcagcc ctgcagtcca agaaccaccc
     2581 ttgaaagtga tccagccaaa tggaaatcag gatgaaagac aagatactag ctcagaagga
     2641 gtatcaaatg tagaggatca gaatgactct gattctacac cgcccaaaaa gaaaatgcgg
     2701 aagacagaaa atggaatgta tgcttgtgat ttgtgtgaca agatattcca aaagagtagt
     2761 tcattattga gacataaata tgaacacaca ggtaaaagac ctcatgagtg tggaatctgt
     2821 aaaaaggcat ttaaacacaa acatcatttg attgaacaca tgcgattaca ttctggagaa
     2881 aagccctatc aatgtgacaa atgtggaaag cgcttctcac actctgggtc ttattctcaa
     2941 cacatgaatc atcgctactc ctactgtaag agagaagcgg aagaacgtga cagcacagag
     3001 caggaagagg cagggcctga aatcctctcg aatgagcacg tgggtgccag ggcgtctccc
     3061 tcacagggcg actcggacga gagagagagt ttgacaaggg aagaggatga agacagtgaa
     3121 aaagaggaag aggaggagga taaagagatg gaagaattgc aggaagaaaa agaatgtgaa
     3181 aaaccacaag gggatgagga agaggaggag gaggaggaag aagtggaaga agaagaggta
     3241 gaagaggcag agaatgaggg agaagaagca aaaactgaag gtctgatgaa ggatgacagg
     3301 gctgaaagtc aagcaagcag cttaggacaa aaagtaggcg agagtagtga gcaagtgtct
     3361 gaagaaaaga caaatgaagc ctagtaatga actagtccgt aacttgaaag tatttcgatt
     3421 tcttggcttt atatatcttg tggaaaggac gacggcgatc gaagtgaggg ctgcta
//
