LOCUS       sequence-281168-        3704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0107
FEATURES             Location/Qualifiers
     source          1..3704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggtgacca gcaactgtac aagaccaacc atgtggccca tggtagtgag
       61 aaccttttct accaacagcc accacttggc gtccacagcg ggctgaacca caactatggg
      121 aatgcagtta cagggggcgg aatggatgcc cctcaggcct cgccaatctc cccccacttc
      181 cctcaagata cacgggatgg tctgggcttg cctgttggct ccaaaaacct tggccaaatg
      241 gatacctcga ggcagggagg gtggggaagt catgcagggc ctggaaacca tgtccagcta
      301 cgtggaaacc tggccaactc aaacatgatg tggggggcac cagcccaggc tgagcccact
      361 gatggctacc aatacaccta ctcccaggcc agcgagatcc ggacccagaa gcttaccagc
      421 ggtgtcttac acaagctgga ctctttcacc caggtgtttg ccaaccaaaa cctgcgaatt
      481 caggtcaaca atatggccca ggtgctgcac actcagtcag cagtgatgga tggagcccct
      541 gacagtgctc tccgccagct gctgtctcag aagcccatgg agcccccagc accggctatc
      601 ccttcccgct accagcaggt gccccagcag cctcaccctg gtttcactgg tgggctgtcc
      661 aaaccagctc ttcaggtcgg gcagcaccct acccaagggc acctgtatta tgactaccag
      721 cagcctctgg ctcaggtgcc agtgcaggga ggacagccac tgcaggcccc acagatgctg
      781 tcacagcaca tgcaacagat gcagcagcac cagtattacc caccgcagca acagcagcaa
      841 gccgggcaac agcgtatctc catgcaagaa atacagacgc agccgcaaca aattcgccca
      901 tcacagccac agccgccgcc acagcagcag cagccgcagc agctacagct gcagcagcgg
      961 cagggttcaa tgcagatacc tcagtattat cagccccaac ccatgatgca gcacttgcaa
     1021 gagcagcagc agcaacagat gcacctgcag cctccttctt atcacaggga ccctcaccag
     1081 tataccccag agcaggcaca cactgtccag ctgattcccc tgggctccat gtcccagtac
     1141 tactaccagg agccccagca gccctacagc caccccctct accagcagag ccacctgtcc
     1201 cagcaccagc agcgtgagga cagtcagctg aagacctact ctagtgacag acaggcccag
     1261 gccatgctga gctcccatgg ggacctgggg cctcctgaca caggaatggg agacccagcg
     1321 agctcagatc tgacccgggt cagcagcacc ctcccccatc gccccctcct atcccccagt
     1381 gggatccacc tcaacaacat ggggcctcag catcagcagc tgtctcccag tgccatgtgg
     1441 ccccagatgc acctacctga tgggagagcc cagccagggt cccctgagtc aagtggccaa
     1501 cccaaaggag cgtttgggga gcagtttgat gccaagaaca agctgacatg ctccatctgc
     1561 ctgaaggagt tcaagaacct gcctgccctg aatggccaca tgcggtccca cgggggaatg
     1621 agggcctccc ccaacctcaa acaggaggaa ggagagaagg tcctgccgcc tcagccccag
     1681 ccaccactgc cgcctccgcc tccgcctccg ccgccaccac agctccctcc cgaggcagaa
     1741 agcctcacgc ctatggtcat gcccgtgtct gtccctgtca agcttctccc gcccaagccc
     1801 agctctcagg ggttcaccaa cagcaccgtt gccgccccct ccgccagaga caagccagcc
     1861 agctcgatgt cggacgacga gatgcctgtg ctcgaaatcc ccaggaagca tcagccgagt
     1921 gtgcccaaag ccgaggagcc cctcaagacc gtgcaggaga agaaaaagtt ccggcaccgg
     1981 ccggaacctc tcttcatccc gccgccgccc tcctacaacc cgaaccccgc tgcctcctac
     2041 tcgggcgcca ccctgtacca gagccagctg cgctccccgc gcgtcctcgg ggaccacctg
     2101 ctcctggacc ccacccacga gctgccccct tacacgcccc cacccatgct gagcccggtg
     2161 cgccagggct cggggctctt cagcaatgtc ctcatctccg gccacggccc tggcgcccac
     2221 ccgcagctgc ccctgacgcc cctgacgccc acaccacggg tgctgctgtg tcgctccaac
     2281 agcatcgatg gcagcaacgt gacggtcacc ccagggcctg gagagcagac tgtagatgtt
     2341 gaaccacgca tcaacattgg cttgagattc caagcagaaa tccctgaact ccaagatatc
     2401 tctgccctgg cccaggacac acacaaggcc acactggtat ggaagccctg gccagaacta
     2461 gaaaaccatg acctccagca aagagtggag aatcttctga atttgtgctg ttccagtgca
     2521 ttgccaggtg gagggaccaa ttctgaattt gctttgcact ctctgtttga ggccaaaggt
     2581 gatgtgatgg ttgctctgga aatgctgcta ctgcggaagc ctgtcaggtt aaaatgtcat
     2641 cctttagcaa attaccacta tgccggttcg gacaagtgga cctccctaga aagaaaactg
     2701 tttaacaaag cactagccac ttacagcaaa gactttattt ttgtacagaa gatggtgaag
     2761 tccaagacgg tggctcagtg cgtggagtac tactacacgt ggaaaaagat catgcggctg
     2821 gggcggaaac accggacacg cctggcagaa atcatcgacg attgtgtgac aagtgaagaa
     2881 gaagaagagt tagaggagga ggaggaggag gacccggaag aagataggaa atccacaaaa
     2941 gaagaagaga gtgaggtgcc gaagtccccg gagccaccac ccgtccccgt cctggctccc
     3001 acggaggggc cgcccctgca ggccctgggc cagccctcag gctccttcat ctgtgaaatg
     3061 cccaactgtg gggctgtgtt cagctcccga caggcactga atggccatgc ccgcatccac
     3121 gggggcacca accaggtgac caaggcccga ggtgccatcc cctctgggaa gcagaagcct
     3181 ggtggcaccc agagtgggta ctgttcggta aagagctcac cctctcacag caccaccagc
     3241 ggcgagacag accccaccac catcttcccc tgcaaggagt gtggcaaagt cttcttcaag
     3301 atcaaaagcc gaaatgcaca catgaaaact cacaggcagc aggaggaaca acagaggcaa
     3361 aaggctcaga aggcggcttt tgcagctgag atggcagcca cgattgagag gactacgggg
     3421 cccgtggggg cgccggggct gctgcccctg gaccagctga gtctgatcaa acccatcaag
     3481 gatgtggaca tcctcgacga cgacgtcgtc cagcagttgg gaggtgtcat ggaagaggct
     3541 gaagttgtgg acaccgatct tctcttggat gatcaagatt cagtcttgct tcagggtgac
     3601 gcagaactat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     3661 atatcttgtg gaaaggacga cggctgcact tcacgttctc ctaa
//
