LOCUS       sequence-281201-        2555 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0354
FEATURES             Location/Qualifiers
     source          1..2555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgaatgc aacccgacga gggagtgggg tagaacagaa gtcccaggag
       61 tcagtatcat ttaaagatgt gactgtgggc ttcacccagg aggagtggca gcacctggac
      121 cctagtcaga gggctctgta tagagatgtg atgctggaga actacagcaa ccttgtctca
      181 gtggggtatt gtgttcacaa accagaggtg atcttcaggc tgcaacaagg agaagagcca
      241 tggaaacagg aggaagaatt cccaagccaa agctttccag tctggacagc tgatcacctg
      301 aaagagagga gccaagaaaa ccaatctaaa catttgtggg aagttgtatt catcaataat
      361 gaaatgctga ctaaggaaca aggtgatgta ataggaatac catttaatgt ggatgtaagt
      421 tcttttcctt ccagaaaaat gttctgtcag tgtgattcat gtggaatgag tttcaacact
      481 gtttcagaat tggttatcag taagataaac tatttaggaa aaaagtctga tgaatttaat
      541 gcctgtggga aattgttact caatattaag catgatgaaa ctcatactca agagaaaaat
      601 gaagttttga aaaataggaa cacactgagt catcatgagg agactttgca gcatgagaag
      661 attcaaactt tagagcacaa ttttgaatac agtatatgtc aggaaaccct ccttgaaaag
      721 gcagtattca atacacagaa gagagagaac gcagaagaga ataactgtga ttataatgaa
      781 tttgggagaa ctttgtgtga tagttcatcc ctcttgttcc atcagatatc tccgtcaagg
      841 gacaatcact atgaatttag tgattgtgag aagttcttat gtgtgaagtc caccctttct
      901 aaacctcatg gggtatctat gaaacactat gattgtggtg aaagtgggaa taatttcagg
      961 aggaaattgt gtctgtcaca ccttcagaaa ggtgataaag gagagaaaca ctttgaatgt
     1021 aatgaatgtg ggaaagcttt ctgggagaag tcacatctca ctcgacatca gagggtgcac
     1081 acaggacaga aaccctttca atgtaatgaa tgtgaaaaag ctttctggga taagtcaaac
     1141 ctcactaaac atcaaagatc acacacaggg gagaaacctt ttgaatgcaa tgaatgtggg
     1201 aaagccttta gccataagtc agccctcaca ttacaccaga gaacacatac aggggagaaa
     1261 ccctatcaat gtaatgcgtg tgggaaaact ttttgccaga aatctgacct cactaaacat
     1321 cagagaacac acacagggct gaaaccctat gaatgttatg aatgtggaaa atccttccgt
     1381 gtgacttcgc accttaaagt acaccagaga actcacacag gtgagaaacc ttttgaatgt
     1441 cttgagtgtg ggaaatcctt tagtgaaaag tcaaatctta cacagcatca gagaattcac
     1501 ataggagata aatcttatga atgtaatgca tgtgggaaaa ctttctacca caagtcatta
     1561 ctcaccaggc atcagataat tcatacaggg tggaaacctt atgaatgtta tgaatgtggg
     1621 aaaaccttct gcttgaagtc agacctcaca gtacatcaga gaacacacac agggcagaaa
     1681 ccctttgcat gtcccgaatg tgggaaattc tttagccata agtcaaccct ctctcaacat
     1741 tatagaacac acacagggga gaaaccctac gaatgtcatg aatgtggaaa aatcttttac
     1801 aataaatcat acctaactaa acataataga acacatacag gggagaaacc ctatgaatgt
     1861 aatgaatgtg gaaaagcctt ctaccagaag tcacaactca ctcagcatca gagaattcac
     1921 ataggggaga aaccctataa atgtaatgag tgtggaaaag ctttctgcca taagtcagct
     1981 ctaattgtac atcagagaac ccatacacaa gaaaagccct ataaatgtaa tgaatgtgga
     2041 aaatctttct gtgtaaaatc aggacttatt ttccatgaga gaaagcacac gggggagaaa
     2101 ccctatgaat gcaatgaatg tgggaaattc ttcaggcaca aatcatcact cacagtacat
     2161 cacagggctc acacaggaga gaaatcttgt caatgtaatg aatgtggaaa aatcttttac
     2221 cgtaaatcgg aacttgctca acatcagaga tcacatacag gggaaaagcc ctatgaatgt
     2281 aacacatgca ggaaaacttt ctctcaaaag tcaaatctca ttgtacatca gagaagacat
     2341 ataggagaaa accttatgaa tgaaatggat attagaaatt tccagccaca agtcagcctc
     2401 cataatgcct cagagtattc acactgtgga gaaagccctg atgacatcct gaatgttcag
     2461 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2521 ggaaaggacg acatgaagga cgttagtaaa tgcat
//
