LOCUS       sequence-28121-e         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M
  TITLE     Genetically encoded FRET sensors to monitor intracellular Zn(2+) 
            homeostasis.
  JOURNAL   Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368.
  PUBMED    19718032
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2009-10"; volume: "6"; issue: 
            "10"; pages: "737-40"
COMMENT     Sequence Label: peCALWY-6
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(463..484)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aggagttagt atgacattga cctgcccaac aagaaggtct gcattgaatc tgagcacagc
       61 atggacactc tgcttgcaac cctgaagaaa acaggaaaga ctgtttccta ccttggcctt
      121 gagctcattc gtggcggatc cggcggcagc ggcggatccg gtggaagcgg tggatccggg
      181 ggtccgcgga tgcagggcac atgcagtacc actctgattg ccattgccgg catgacctgt
      241 gcatccagtg tccattccat tgaaggcatg atctcccaac tggaaggggt gcagcaaata
      301 tcggtgtctt tggccgaagg gactgcaaca gttctttata atcccgctgt aattagccca
      361 gaagaactca gagctgctat agaagacatg ggatttgagg cttcagtctc ggtacccatg
      421 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      481 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      541 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      601 gtgaccacct tcggctacgg cctgatgtgc ttcgcccgct accccgacca catgaagcag
      661 cacgacttct tcaag
//
