LOCUS       sequence-281282-        1412 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0581
FEATURES             Location/Qualifiers
     source          1..1412
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggactcagt ggcctttgag gatgtggctg tgaacttcac ccaggaggag
       61 tgggctttgc tgagtccttc ccagaagaat ctctacagag atgtgacgct ggaaaccttc
      121 aggaacctgg cctcggtcgg aatccaatgg aaagaccagg acattgagaa tctgtaccaa
      181 aacctgggga ttaagctaag aagtctggtg gagagactct gtggacgtaa agaagggaat
      241 gaacacagag aaactttcag ccagattcct gattgtcacc tgaacaagaa aagtcaaact
      301 ggagtgaaac catgcaaatg cagcgtgtgt gggaaagtct tcctccgtca ttcattcctg
      361 gacaggcaca tgagagctca tgctggacac aaacgatctg agtgtggtgg ggaatggaga
      421 gagacgcccc gtaaacagaa acaacatggg aaagcctcca tttcccccag tagtggtgca
      481 cggcgcacag taacaccaac tcgaaagaga ccttatgaat gcaaggtgtg cgggaaagcc
      541 tttaattctc ccaatttatt tcaaatccat caaagaactc acactggaaa gaggtcctat
      601 aaatgtaggg aaatagtgag agccttcaca gtttccagtt tctttcgaaa acatggaaaa
      661 atgcatactg gagaaaaacg ctatgaatgt aaatactgtg gaaaacctat cgattatccc
      721 agtttatttc aaattcatgt tagaactcac actggagaaa aaccttacaa atgtaaacaa
      781 tgtggtaaag ccttcatttc cgcaggttac cttcggacac atgaaatcag atctcacgcg
      841 ctggagaaat cccaccaatg tcaggaatgt gggaaaaaac tcagttgttc cagttccctt
      901 cacagacatg aaagaactca tagtggagga aaactctacg aatgtcaaaa atgtgccaaa
      961 gtctttagat gtcccacgtc ccttcaagca catgaaagag ctcacactgg agaaagacct
     1021 tatgaatgta ataaatgtgg taaaaccttc aattatccca gttgttttcg aagacataaa
     1081 aaaactcata gtggagaaaa gccatatgaa tgtacaaggt gtggtaaagc ctttgggtgg
     1141 tgcagttccc tccgaagaca tgaaatgact cacactggag aaaaaccctt tgattgtaaa
     1201 cagtgtggta aagtctttac tttttcaaat taccttagac ttcatgaaag aactcatttg
     1261 gccgggcgta gccagtgctt tggcaggagg cagggggatc acctgagccc aggagtttag
     1321 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1381 aaggacgagc gatgacgtac atatatgaac gt
//
