LOCUS       sequence-28135-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Drinnenberg IA, Weinberg DE, Xie KT, Mower JP, Wolfe KH, Fink GR, 
            Bartel DP
  TITLE     RNAi in budding yeast.
  JOURNAL   Science. 2009 Oct 23;326(5952):544-550. doi: 
            10.1126/science.1176945. Epub 2009 Sep 10.
  PUBMED    19745116
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1176945"; journalName: "Science"; date: 
            "2009-10-23- 23"; volume: "326"; issue: "5952"; pages: "544-550"
COMMENT     Sequence Label: pYES2.1-GFP
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          96..325
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     308..327
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             412..453
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             463..480
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      501..748
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(517..535)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(825..842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agctgtacag aagctttaag aattcagtgg atccactagt aacggccgcc agtgtgctgg
       61 aattaattcg ctgtctgcga gggccagctg ttggggtgag tactccctct caaaagcggg
      121 catgacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg
      181 gcccgcggtg atgcctttga gggtggccgc gtccatctgg tcagaaaaga caatcttttt
      241 gttgtcaagc ttgaggtgtg gcaggcttga gatctggcca tacacttgag tgacaatgac
      301 atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca ggtcgagcat
      361 gcatctaggg cggccaattc caagggcgag cttcgaggtc acccattcga aggtaagcct
      421 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat
      481 tgagtttcta gagggccgca tcatgtaatt agttatgtca cgcttacatt cacgccctcc
      541 ccccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat
      601 ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt
      661 ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt
      721 tttgggacgc tcgaaggctt taatttgcaa gctgcggccc tgcattaatg aatcggccaa
      781 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      841 ctgcgctcgg tcgttcggct gcggcgag
//
