LOCUS       sequence-281360-        7769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0757
FEATURES             Location/Qualifiers
     source          1..7769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgccgccgct cctggcgccc ctgctctgcc tggcgctgct gcccgcgctc
       61 gccgcacgag gcccgcgatg ctcccagccc ggtgagacct gcctgaatgg cgggaagtgt
      121 gaagcggcca atggcacgga ggcctgcgtc tgtggcgggg ccttcgtggg cccgcgatgc
      181 caggacccca acccgtgcct cagcaccccc tgcaagaacg ccgggacatg ccacgtggtg
      241 gaccgcagag gcgtggcaga ctatgcctgc agctgtgccc tgggcttctc tgggcccctc
      301 tgcctgacac ccctggacaa tgcctgcctc accaacccct gccgcaacgg gggcacctgc
      361 gacctgctca cgctgacgga gtacaagtgc cgctgcccgc ccggctggtc agggaaatcg
      421 tgccagcagg ctgacccgtg cgcctccaac ccctgcgcca acggtggcca gtgcctgccc
      481 ttcgaggcct cctacatctg ccactgccca cccagcttcc atggccccac ctgccggcag
      541 gatgtcaacg agtgtggcca gaagcccggg ctttgccgcc acggaggcac ctgccacaac
      601 gaggtcggct cctaccgctg cgtctgccgc gccacccaca ctggccccaa ctgcgagcgg
      661 ccctacgtgc cctgcagccc ctcgccctgc cagaacgggg gcacctgccg ccccacgggc
      721 gacgtcaccc acgagtgtgc ctgcctgcca ggcttcaccg gccagaactg tgaggaaaat
      781 atcgacgatt gtccaggaaa caactgcaag aacgggggtg cctgtgtgga cggcgtgaac
      841 acctacaact gccgctgccc gccagagtgg acaggtcagt actgtaccga ggatgtggac
      901 gagtgccagc tgatgccaaa tgcctgccag aacggcggga cctgccacaa cacccacggt
      961 ggctacaact gcgtgtgtgt caacggctgg actggtgagg actgcagcga gaacattgat
     1021 gactgtgcca gcgccgcctg cttccacggc gccacctgcc atgaccgtgt ggcctccttc
     1081 tactgcgagt gtccccatgg ccgcacaggt ctgctgtgcc acctcaacga cgcatgcatc
     1141 agcaacccct gtaacgaggg ctccaactgc gacaccaacc ctgtcaatgg caaggccatc
     1201 tgcacctgcc cctcggggta cacgggcccg gcctgcagcc aggacgtgga tgagtgctcg
     1261 ctgggtgcca acccctgcga gcatgcgggc aagtgcatca acacgctggg ctccttcgag
     1321 tgccagtgtc tgcagggcta cacgggcccc cgatgcgaga tcgacgtcaa cgagtgcgtc
     1381 tcgaacccgt gccagaacga cgccacctgc ctggaccaga ttggggagtt ccagtgcatc
     1441 tgcatgcccg gctacgaggg tgtgcactgc gaggtcaaca cagacgagtg tgccagcagc
     1501 ccctgcctgc acaatggccg ctgcctggac aagatcaatg agttccagtg cgagtgcccc
     1561 acgggcttca ctgggcatct gtgccagtac gatgtggacg agtgtgccag caccccctgc
     1621 aagaatggtg ccaagtgcct ggacggaccc aacacttaca cctgtgtgtg cacggaaggg
     1681 tacacgggga cgcactgcga ggtggacatc gatgagtgcg accccgaccc ctgccactac
     1741 ggctcctgca aggacggcgt cgccaccttc acctgcctct gccgcccagg ctacacgggc
     1801 caccactgcg agaccaacat caacgagtgc tccagccagc cctgccgcca cgggggcacc
     1861 tgccaggacc gcgacaacgc ctacctctgc ttctgcctga aggggaccac aggacccaac
     1921 tgcgagatca acctggatga ctgtgccagc agcccctgcg actcgggcac ctgtctggac
     1981 aagatcgatg gctacgagtg tgcctgtgag ccgggctaca cagggagcat gtgtaacatc
     2041 aacatcgatg agtgtgcggg caacccctgc cacaacgggg gcacctgcga ggacggcatc
     2101 aatggcttca cctgccgctg ccccgagggc taccacgacc ccacctgcct gtctgaggtc
     2161 aatgagtgca acagcaaccc ctgcgtccac ggggcctgcc gggacagcct caacgggtac
     2221 aagtgcgact gtgaccctgg gtggagtggg accaactgtg acatcaacaa caatgagtgt
     2281 gaatccaacc cttgtgtcaa cggcggcacc tgcaaagaca tgaccagtgg ctacgtgtgc
     2341 acctgccggg agggcttcag cggtcccaac tgccagacca acatcaacga gtgtgcgtcc
     2401 aacccatgtc tgaaccaggg cacgtgtatt gacgacgttg ccgggtacaa gtgcaactgc
     2461 ctgctgccct acacaggtgc cacgtgtgag gtggtgctgg ccccgtgtgc ccccagcccc
     2521 tgcagaaacg gcggggagtg caggcaatcc gaggactatg agagcttctc ctgtgtctgc
     2581 cccacgggct ggcaagggca gacctgtgag gtcgacatca acgagtgcgt tctgagcccg
     2641 tgccggcacg gcgcatcctg ccagaacacc cacggcggct accgctgcca ctgccaggcc
     2701 ggctacagtg ggcgcaactg cgagaccgac atcgacgact gccggcccaa cccgtgtcac
     2761 aacgggggct cctgcacaga cggcatcaac acggccttct gcgactgcct gcccggcttc
     2821 cggggcactt tctgtgagga ggacatcaac gagtgtgcca gtgacccctg ccgcaacggg
     2881 gccaactgca cggactgcgt ggacagctac acgtgcacct gccccgcagg cttcagcggg
     2941 atccactgtg agaacaacac gcctgactgc acagagagct cctgcttcaa cggtggcacc
     3001 tgcgtggacg gcatcaactc gttcacctgc ctgtgtccac ccggcttcac gggcagctac
     3061 tgccagcacg atgtcaatga gtgcgactca cagccctgcc tgcatggcgg cacctgtcag
     3121 gacggctgcg gctcctacag gtgcacctgc ccccagggct acactggccc caactgccag
     3181 aaccttgtgc actggtgtga ctcctcgccc tgcaagaacg gcggcaaatg ctggcagacc
     3241 cacacccagt accgctgcga gtgccccagc ggctggaccg gcctttactg cgacgtgccc
     3301 agcgtgtcct gtgaggtggc tgcgcagcga caaggtgttg acgttgcccg cctgtgccag
     3361 catggagggc tctgtgtgga cgcgggcaac acgcaccact gccgctgcca ggcgggctac
     3421 acaggcagct actgtgagga cctggtggac gagtgctcac ccagcccctg ccagaacggg
     3481 gccacctgca cggactacct gggcggctac tcctgcaagt gcgtggccgg ctaccacggg
     3541 gtgaactgct ctgaggagat cgacgagtgc ctctcccacc cctgccagaa cgggggcacc
     3601 tgcctcgacc tccccaacac ctacaagtgc tcctgcccac ggggcactca gggtgtgcac
     3661 tgtgagatca acgtggacga ctgcaatccc cccgttgacc ccgtgtcccg gagccccaag
     3721 tgctttaaca acggcacctg cgtggaccag gtgggcggct acagctgcac ctgcccgccg
     3781 ggcttcgtgg gtgagcgctg tgagggggat gtcaacgagt gcctgtccaa tccctgcgac
     3841 gcccgtggca cccagaactg cgtgcagcgc gtcaatgact tccactgcga gtgccgtgct
     3901 ggtcacaccg ggcgccgctg cgagtccgtc atcaatggct gcaaaggcaa gccctgcaag
     3961 aatgggggca cctgcgccgt ggcctccaac accgcccgcg ggttcatctg caagtgccct
     4021 gcgggcttcg agggcgccac gtgtgagaat gacgctcgta cctgcggcag cctgcgctgc
     4081 ctcaacggcg gcacatgcat ctccggcccg cgcagcccca cctgcctgtg cctgggcccc
     4141 ttcacgggcc ccgaatgcca gttcccggcc agcagcccct gcctgggcgg caacccctgc
     4201 tacaaccagg ggacctgtga gcccacatcc gagagcccct tctaccgttg cctgtgcccc
     4261 gccaaattca acgggctctt gtgccacatc ctggactaca gcttcggggg tggggccggg
     4321 cgcgacatcc ccccgccgct gatcgaggag gcgtgcgagc tgcccgagtg ccaggaggac
     4381 gcgggcaaca aggtctgcag cctgcagtgc aacaaccacg cgtgcggctg ggacggcggt
     4441 gactgctccc tcaacttcaa tgacccctgg aagaactgca cgcagtctct gcagtgctgg
     4501 aagtacttca gtgacggcca ctgtgacagc cagtgcaact cagccggctg cctcttcgac
     4561 ggctttgact gccagcgtgc ggaaggccag tgcaaccccc tgtacgacca gtactgcaag
     4621 gaccacttca gcgacgggca ctgcgaccag ggctgcaaca gcgcggagtg cgagtgggac
     4681 gggctggact gtgcggagca tgtacccgag aggctggcgg ccggcacgct ggtggtggtg
     4741 gtgctgatgc cgccggagca gctgcgcaac agctccttcc acttcctgcg ggagctcagc
     4801 cgcgtgctgc acaccaacgt ggtcttcaag cgtgacgcac acggccagca gatgatcttc
     4861 ccctactacg gccgcgagga ggagctgcgc aagcacccca tcaagcgtgc cgccgagggc
     4921 tgggccgcac ctgacgccct gctgggccag gtgaaggcct cgctgctccc tggtggcagc
     4981 gagggtgggc ggcggcggag ggagctggac cccatggacg tccgcggctc catcgtctac
     5041 ctggagattg acaaccggca gtgtgtgcag gcctcctcgc agtgcttcca gagtgccacc
     5101 gacgtggccg cattcctggg agcgctcgcc tcgctgggca gcctcaacat cccctacaag
     5161 atcgaggccg tgcagagtga gaccgtggag ccgcccccgc cggcgcagct gcacttcatg
     5221 tacgtggcgg cggccgcctt tgtgcttctg ttcttcgtgg gctgcggggt gctgctgtcc
     5281 cgcaagcgcc ggcggcagca tggccagctc tggttccctg agggcttcaa agtgtctgag
     5341 gccagcaaga agaagcggcg ggagcccctc ggcgaggact ccgtgggcct caagcccctg
     5401 aagaacgctt cagacggtgc cctcatggac gacaaccaga atgagtgggg ggacgaggac
     5461 ctggagacca agaagttccg gttcgaggag cccgtggttc tgcctgacct ggacgaccag
     5521 acagaccacc ggcagtggac tcagcagcac ctggatgccg ctgacctgcg catgtctgcc
     5581 atggccccca caccgcccca gggtgaggtt gacgccgact gcatggacgt caatgtccgc
     5641 gggcctgatg gcttcacccc gctcatgatc gcctcctgca gcgggggcgg cctggagacg
     5701 ggcaacagcg aggaagagga ggacgcgccg gccgtcatct ccgacttcat ctaccagggc
     5761 gccagcctgc acaaccagac agaccgcacg ggcgagaccg ccttgcacct ggccgcccgc
     5821 tactcacgct ctgatgccgc caagcgcctg ctggaggcca gcgcagatgc caacatccag
     5881 gacaacatgg gccgcacccc gctgcatgcg gctgtgtctg ccgacgcaca aggtgtcttc
     5941 cagatcctga tccggaaccg agccacagac ctggatgccc gcatgcatga tggcacgacg
     6001 ccactgatcc tggctgcccg cctggccgtg gagggcatgc tggaggacct catcaactca
     6061 cacgccgacg tcaacgccgt agatgacctg ggcaagtccg ccctgcactg ggccgccgcc
     6121 gtgaacaatg tggatgccgc agttgtgctc ctgaagaacg gggctaacaa agatatgcag
     6181 aacaacaggg aggagacacc cctgtttctg gccgcccggg agggcagcta cgagaccgcc
     6241 aaggtgctgc tggaccactt tgccaaccgg gacatcacgg atcatatgga ccgcctgccg
     6301 cgcgacatcg cacaggagcg catgcatcac gacatcgtga ggctgctgga cgagtacaac
     6361 ctggtgcgca gcccgcagct gcacggagcc ccgctggggg gcacgcccac cctgtcgccc
     6421 ccgctctgct cgcccaacgg ctacctgggc agcctcaagc ccggcgtgca gggcaagaag
     6481 gtccgcaagc ccagcagcaa aggcctggcc tgtggaagca aggaggccaa ggacctcaag
     6541 gcacggagga agaagtccca ggacggcaag ggctgcctgc tggacagctc cggcatgctc
     6601 tcgcccgtgg actccctgga gtcaccccat ggctacctgt cagacgtggc ctcgccgcca
     6661 ctgctgccct ccccgttcca gcagtctccg tccgtgcccc tcaaccacct gcctgggatg
     6721 cccgacaccc acctgggcat cgggcacctg aacgtggcgg ccaagcccga gatggcggcg
     6781 ctgggtgggg gcggccggct ggcctttgag actggcccac ctcgtctctc ccacctgcct
     6841 gtggcctctg gcaccagcac cgtcctgggc tccagcagcg gaggggccct gaatttcact
     6901 gtgggcgggt ccaccagttt gaatggtcaa tgcgagtggc tgtcccggct gcagagcggc
     6961 atggtgccga accaatacaa ccctctgcgg gggagtgtgg caccaggccc cctgagcaca
     7021 caggccccct ccctgcagca tggcatggta ggcccgctgc acagtagcct tgctgccagc
     7081 gccctgtccc agatgatgag ctaccagggc ctgcccagca cccggctggc cacccagcct
     7141 cacctggtgc agacccagca ggtgcagcca caaaacttac agatgcagca gcagaacctg
     7201 cagccagcaa acatccagca gcagcaaagc ctgcagccgc caccaccacc accacagccg
     7261 caccttggcg tgagctcagc agccagcggc cacctgggcc ggagcttcct gagtggagag
     7321 ccgagccagg cagacgtgca gccactgggc cccagcagcc tggcggtgca cactattctg
     7381 ccccaggaga gccccgccct gcccacgtcg ctgccatcct cgctggtccc acccgtgacc
     7441 gcagcccagt tcctgacgcc cccctcgcag cacagctact cctcgcctgt ggacaacacc
     7501 cccagccacc agctacaggt gcctgagcac cccttcctca ccccgtcccc tgagtcccct
     7561 gaccagtggt ccagctcgtc cccgcattcc aacgtctccg actggtccga gggcgtctcc
     7621 agccctccca ccagcatgca gtcccagatc gcccgcattc cggaggcctt caagtagtaa
     7681 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     7741 gacgagctcc acgtgcccac cagagaccc
//
