LOCUS       sequence-281434-       12406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12406)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1346
FEATURES             Location/Qualifiers
     source          1..12406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8263..8851
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8316..8336)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8734..8745)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8923..9156
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9234..9355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9271..9290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9325..9344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9395..9530
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9550..9569)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9551..9569)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9579..9596)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9579..9595)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9588..9610)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9737..10192
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9824..9843)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10034..10055
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10218..10322
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10323..10391,10392..11183)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10541..10560)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11354..11942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11843..11862
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12096..12113
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12230..12251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12266..12271,12272..12289,12290..12296)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12304..12320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12309..12331
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12328..12344
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12328..12344
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12363..12383
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12365..12383
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggac cagtacagcc ttggagacga
     4141 gggtgccctc ccatcagaaa tgcacctccc ttcattttca gagagccaag ggctcaactg
     4201 cagcgacacc ctcaaccggg atttgggtcc caacacgcga ggctttcttt atgctggcct
     4261 gagtggtctg gacccggacc ccagcctccc aacgcctgac atgtccagcg aggtgctgga
     4321 ggacaactta gacaccttgt ccctgtactc cgggaaggac agtgattctg tgaagctgct
     4381 ggaggagtac gcagattcgg agtctcaggc atccttacaa gacctggggc taggtgtgct
     4441 taaggctaaa gaggctgacg aaggaggaag ggccacctcg gggagtgcca ggaaaggaaa
     4501 gcggcagcac agttcccctc aaaacccact tctggactgc agcctgtgcg ggaaggtgtt
     4561 cagcagcgcc agttctctga gcaagcacta cctgacacac agccaggaaa ggaagcacgt
     4621 ctgcaaaatc tgcagcaagg cctttaagcg ccaggaccac ctgaccgggc acatgctcac
     4681 ccaccagaag acaaagccct tcgtgtgcat cgagcagggc tgcagcaaga gctactgcga
     4741 ctaccgctct ctgcgccggc actacgaggt ccatcacggc ctgtgcatcc tgaaggaagc
     4801 ccccccggag gaagaggcct gcggggactc cccccacgcc cacgagtcgg ccggccagcc
     4861 gccccccagc agcctgcggt ccctggtgcc cccagaggcc aggtcccccg gctccctcct
     4921 gccccaccgg gacctcctgc gccgcatcgt gagtagcatc gtccaccaga agaccccttc
     4981 tcctggccca gccccggcgg gggcttcaga cagcgaaggg aggaacactg cctgtccctg
     5041 ccccgcctca tcggggtcct cgtcctgcac cccagccggc ccccacgcgg ccccagcagc
     5101 gctggacacc gagcttcccg aggagccttg cctcccacag aaagagccgg ccactgacgt
     5161 gttcacagcc cctaattcca gggccgccga gaacggcgcc cccgacccgc cagagccgga
     5221 gccagatacc gcgctgctcc aggcccggtc caccgcggag tgctggcccg aaggcggctc
     5281 cgtgcctgcc tgcctgcctc tcttccgagg ccagacggtc cctgccagtt cccagccatc
     5341 gagccacagc ttccagtggc tccggaacct gccgggctgt cccaaaagca aaggcaacaa
     5401 cgtgtttgtt gtccacaagc cctcggccgt gccctcgcgg gagggctccg agtctggccc
     5461 gggacccagc agcggaagcc cctcggagga gtccccgccc ggccccggcg gtggcctgga
     5521 ggatgctctg cccttccctg ccgcgctcct cagggtcccc gcggaggccc cgagcgaccc
     5581 caggtcggcc agcggggaag atgacccctg cgcccccaag aaggtcaagg tcgactgcga
     5641 ctccttcctg tgccagaacc ccggggagcc cggcctccag gaggcccaga aggcaggcgg
     5701 gctccctgcg gatgcctcgc cgctcttccg ccagctcttc ctcaagtcgc aggaacctct
     5761 tgtgagccac gagcagatgc aggtgttcca gatgatcacc aagtcccagc ggatcttctc
     5821 ccatgcccag gtggcagcag tctcctccca gctccctgcg cccgagggca aaccagccgc
     5881 cctgaggccg ctgcaggggc cgtggccgca gcagccccca ccactggctc ctgctgtgga
     5941 ctctctccac gccggccctg gaaaccccga ggcagagggc tccccagccc gcaggagaaa
     6001 aaccacaccc ggggttccca gagaggcctc ccccggcagc acgagacgag acgcaaaggg
     6061 gggactgaaa gtggccgcgg ttccaacgcc ccttgcagca ccgtctctgg acccttccag
     6121 gaatccagac atctcttctc tggccaagca gctgcgatcc tctaaaggga ccttggacct
     6181 ggaggacatc ttcccctcca caggccaacg gcagacccag ttaggtgggg aggagccacc
     6241 aggagcctcg ctgccaggga agcaggcccc agccgagaat ggcgcggctt caaggatcac
     6301 aaaaggtgaa aagggcccag cctgctcccg gggtggaggc taccggctct tgggcaaccc
     6361 cagggccccg cgattctccg gcttccggaa agagaaggcg aagatggata tgtgctgtgc
     6421 ggcttctccg agccaggtag ccatggcctc cttctcatcg gccgggcccc cggcagatcc
     6481 ctccaagtcc aagctgacaa tattcagcag aatccagggt ggaaacatct acaggctccc
     6541 ccatccggtg aaggaagaga atgtggcagg cagaggtaac cagcaaaacg gcagtcccac
     6601 agactggacg aagcccagga gcacttttgt ctgcaagaac tgcagccaga tgttttatac
     6661 ggagaaaggg ctgagcagcc acatgtgttt tcacagcgac cagtggccgt cacctcgagg
     6721 gaagcaggaa ccgcaggtgt ttggcacaga gttttgcaag ccgctaagac aggtgctgag
     6781 gccagaaggg gacaggcata gtcccccagg aaccaagaag cccttggacc ccacagctgc
     6841 agcccctttg gtggtccccc aatcgatccc cgtggttcca gtgacccgac acatagggag
     6901 catggccatg ggacaagaga aagacgggga ggagcgagac agcaaggaga gcagccagca
     6961 gagaaagcgg aagaagcggc ccccaccctc cacggctggg gagcctggcc ctgcaggatg
     7021 ccaccagagc cgcctgcggt cacccatgtt cctggtggac tgcctcctga agggcttatt
     7081 ccagtgctcc ccctacacac cacccccaat gctcagcccc atccgggagg gctctggggt
     7141 gtacttcaac accctctgtt ccacgtccac tcaggccagc cctgaccagc tcatcagctc
     7201 catgctcgat caagtggatg ggtcctttgg catctgtgtg gtgaaggatg acaccaaaat
     7261 cagcattgag ccacatatca atataggaag ccggtttcag gcagagatcc cggaactcca
     7321 ggagagatcc ctggctggaa ctgacgagca tgtagcttct ctggtctgga agccatgggg
     7381 agatatgatg atcagctcag agacacagga tagagtgacc gagctctgca atgtggcatg
     7441 ctccagcgtg atgccaggag ggggcaccaa cctggagctc gctctgcact gcctgcacga
     7501 ggctcagggc aacgttcagg tcgccctgga gactcttctg ctccgagggc cccacaagcc
     7561 acggacacac ctgctcgctg actatcgcta cacaggttca gacgtctgga cccctataga
     7621 gaagaggctt tttaagaagg cgttctatgc ccacaagaag gacttctact tgatacacaa
     7681 gatgatccag acaaagacgg tagctcagtg cgttgagtat tattacatct ggaaaaaaat
     7741 gatcaagttt gactgtggcc gagccccagg gctagaaaag agggtcaaga gagagccgga
     7801 ggaagtggaa aggacagagg aaaaggtccc atgcagccct cgggagagac ccagccacca
     7861 tccaactccc aagttaaaga ccaagagtta taggagggag tccatcctca gctccagccc
     7921 aaatgcaggc tccaagcgga cccccgagct gttgggaagt gcagagagcc agggcatttt
     7981 tccatgcaga gagtgtgaaa gggtgtttga caagatcaag agtcgaaatg cccatatgaa
     8041 gcggcaccgc cttcaggacc acgtggagcc catcatcagg gtgaagtggc cagtgaagcc
     8101 cttccagcta aaggaagagg agctgggagc tgacatcggc cccctgcagt ggtagtaatg
     8161 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     8221 cgatgcttaa tatgccgagc ctcgtatacg cgttaagtcg acaatcaacc tctggattac
     8281 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     8341 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     8401 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     8461 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     8521 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     8581 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     8641 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     8701 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     8761 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     8821 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     8881 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     8941 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     9001 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9061 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9121 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     9181 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     9241 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9301 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9361 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     9421 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9481 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     9541 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     9601 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     9661 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     9721 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     9781 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     9841 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     9901 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     9961 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    10021 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    10081 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    10141 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
    10201 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    10261 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    10321 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    10381 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    10441 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    10501 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    10561 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    10621 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    10681 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    10741 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    10801 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    10861 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    10921 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10981 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    11041 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    11101 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    11161 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    11221 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    11281 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    11341 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    11401 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    11461 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    11521 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    11581 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    11641 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    11701 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    11761 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    11821 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    11881 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    11941 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    12001 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    12061 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    12121 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    12181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    12241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    12301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    12361 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
