LOCUS       sequence-281474-        1535 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1419
FEATURES             Location/Qualifiers
     source          1..1535
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagtggag gaataagaaa aggagcgatt ggctgtcgat ggtgctcaga
       61 actgctggag tggaggaagc ctttgggtct gaagtgtctg tgagacctca cagaagagca
      121 cccctgggct ccacttacct gccccctgct ccttcaggga tggaggcaat ggcggccagc
      181 acttccctgc ctgaccctgg agactttgac cggaacgtgc cccggatctg tggggtgtgt
      241 ggagaccgag ccactggctt tcacttcaat gctatgacct gtgaaggctg caaaggcttc
      301 ttcaggcgaa gcatgaagcg gaaggcacta ttcacctgcc ccttcaacgg ggactgccgc
      361 atcaccaagg acaaccgacg ccactgccag gcctgccggc tcaaacgctg tgtggacatc
      421 ggcatgatga aggagttcat tctgacagat gaggaagtgc agaggaagcg ggagatgatc
      481 ctgaagcgga aggaggagga ggccttgaag gacagtctgc ggcccaagct gtctgaggag
      541 cagcagcgca tcattgccat actgctggac gcccaccata agacctacga ccccacctac
      601 tccgacttct gccagttccg gcctccagtt cgtgtgaatg atggtggagg gagccatcct
      661 tccaggccca actccagaca cactcccagc ttctctgggg actcctcctc ctcctgctca
      721 gatcactgta tcacctcttc agacatgatg gactcgtcca gcttctccaa tctggatctg
      781 agtgaagaag attcagatga cccttctgtg accctagagc tgtcccagct ctccatgctg
      841 ccccacctgg ctgacctggt cagttacagc atccaaaagg tcattggctt tgctaagatg
      901 ataccaggat tcagagacct cacctctgag gaccagatcg tactgctgaa gtcaagtgcc
      961 attgaggtca tcatgttgcg ctccaatgag tccttcacca tggacgacat gtcctggacc
     1021 tgtggcaacc aagactacaa gtaccgcgtc agtgacgtga ccaaagccgg acacagcctg
     1081 gagctgattg agcccctcat caagttccag gtgggactga agaagctgaa cttgcatgag
     1141 gaggagcatg tcctgctcat ggccatctgc atcgtctccc cagatcgtcc tggggtgcag
     1201 gacgccgcgc tgattgaggc catccaggac cgcctgtcca acacactgca gacgtacatc
     1261 cgctgccgcc acccgccccc gggcagccac ctgctctatg ccaagatgat ccagaagcta
     1321 gccgacctgc gcagcctcaa tgaggagcac tccaagcagt accgctgcct ctccttccag
     1381 cctgagtgca gcatgaagct aacgcccctt gtgctcgaag tgtttggcaa tgagatctcc
     1441 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1501 ggaaaggacg ataatgcttt gagtctgggt gtgac
//
