LOCUS       sequence-281483-        3068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1429
FEATURES             Location/Qualifiers
     source          1..3068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagaccaa aggctaccac agtctccctg aaggtctaga tatggaaaga
       61 cggtggggtc aagtttctca ggctgtggag cgttcttccc tgggacctac agagaggacc
      121 gatgagaata actacatgga gattgtcaac gtaagctgtg tttccggtgc tattccaaac
      181 aacagtactc aaggaagcag caaagaaaaa caagaactac tcccttgcct tcagcaagac
      241 aataatcggc ctgggatttt aacatctgat attaaaactg agctggaatc taaggaactt
      301 tcagcaactg tagctgagtc catgggttta tatatggatt ctgtaagaga tgctgactat
      361 tcctatgagc agcagaacca acaaggaagc atgagtccag ctaagattta tcagaatgtt
      421 gaacagctgg tgaaatttta caaaggaaat ggccatcgtc cttccactct aagttgtgtg
      481 aacacgccct tgagatcatt tatgtctgac tctgggagct ccgtgaatgg tggcgtcatg
      541 cgcgccgttg ttaaaagccc tatcatgtgt catgagaaaa gcccgtctgt ttgcagccct
      601 ctgaacatga catcttcggt ttgcagccct gctggaatca actctgtgtc ctccaccaca
      661 gccagctttg gcagttttcc agtgcacagc ccaatcaccc agggaactcc tctgacatgc
      721 tcccctaatg ttgaaaatcg aggctccagg tcgcacagcc ctgcacatgc tagcaatgtg
      781 ggctctcctc tctcaagtcc gttaagtagc atgaaatcct caatttccag ccctccaagt
      841 cactgcagtg taaaatctcc agtctccagt cccaataatg tcactctgag atcctctgtg
      901 tctagccctg caaatattaa caactcaagg tgctctgttt ccagcccttc gaacactaat
      961 aacagatcca cgctttccag tccggcagcc agtactgtgg gatctatctg tagccctgta
     1021 aacaatgcct tcagctacac tgcttctggc acctctgctg gatccagtac attgcgggat
     1081 gtggttccca gtccagacac gcaggagaaa ggtgctcaag aggtcccttt tcctaagact
     1141 gaggaagtag agagtgccat ctcaaatggt gtgactggcc agcttaatat tgtccagtac
     1201 ataaaaccag aaccagatgg agcttttagc agctcatgtc taggaggaaa tagcaaaata
     1261 aattcggatt cttcattctc agtaccaata aagcaagaat caaccaagca ttcatgttca
     1321 ggcacctctt ttaaagggaa tccaacagta aacccgtttc catttatgga tggctcgtat
     1381 ttttccttta tggatgataa agactattat tccctatcag gaattttagg accacctgtg
     1441 cccggctttg atggtaactg tgaaggcagc ggattcccag tgggtattaa acaagaacca
     1501 gatgatggga gctattaccc agaggccagc atcccttcct ctgctattgt tggggtgaat
     1561 tcaggtggac agtccttcca ctacaggatt ggtgctcaag gtacaatatc tttatcacga
     1621 tcggctagag accaatcttt ccaacacctg agttcctttc ctcctgtcaa tactttagtg
     1681 gagtcatgga aatcacacgg cgacctgtcg tctagaagaa gtgatgggta tccggtctta
     1741 gaatacattc cagaaaatgt atcaagctct actttacgaa gtgtttctac tggatcttca
     1801 agaccttcaa aaatatgttt ggtgtgtggg gatgaggctt caggatgcca ttatggggta
     1861 gtcacctgtg gcagctgcaa agttttcttc aaaagagcag tggaaggtaa atgttcatgg
     1921 caacacaact atttatgtgc tggaagaaat gattgcatca ttgataagat tcgacgaaag
     1981 aattgtcctg cttgcagact tcagaaatgt cttcaagctg gaatgaattt aggagcacga
     2041 aagtcaaaga agttgggaaa gttaaaaggg attcacgagg agcagccaca gcagcagcag
     2101 cccccacccc cacccccacc cccgcaaagc ccagaggaag ggacaacgta catcgctcct
     2161 gcaaaagaac cctcggtcaa cacagcactg gttcctcagc tctccacaat ctcacgagcg
     2221 ctcacacctt cccccgttat ggtccttgaa aacattgaac ctgaaattgt atatgcaggc
     2281 tatgacagct caaaaccaga tacagccgaa aatctgctct ccacgctcaa ccgcttagca
     2341 ggcaaacaga tgatccaagt cgtgaagtgg gcaaaggtac ttccaggatt taaaaacttg
     2401 cctcttgagg accaaattac cctaatccag tattcttgga tgtgtctatc atcatttgcc
     2461 ttgagctgga gatcgtacaa acatacgaac agccaatttc tctattttgc accagaccta
     2521 gtctttaatg aagagaagat gcatcagtct gccatgtatg aactatgcca ggggatgcac
     2581 caaatcagcc ttcagttcgt tcgactgcag ctcacctttg aagaatacac catcatgaaa
     2641 gttttgctgc tactaagcac aattccaaag gatggcctca aaagccaggc tgcatttgaa
     2701 gaaatgagga caaattacat caaagaactg aggaagatgg taactaagtg tcccaacaat
     2761 tctgggcaga gctggcagag gttctaccaa ctgaccaagc tgctggactc catgcatgac
     2821 ctggtgagcg acctgctgga attctgcttc tacaccttcc gagagtccca tgcgctgaag
     2881 gtagagttcc ccgcaatgct ggtggagatc atcagcgacc agctgcccaa ggtggagtcg
     2941 gggaacgcca agccgctcta cttccaccgg aagtagtaat gaactagtcc gtaacttgaa
     3001 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaatctc agtcttagtc
     3061 ggcggaga
//
